Secondary metabolite content in <i>Fabiana imbricata</i> plants and <i>in vitro</i> cultures

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dc.contributor.authorSchmeda-Hirschmann, G
dc.contributor.authorJordan, M
dc.contributor.authorGerth, A
dc.contributor.authorWilken, D
dc.contributor.authorHormazabal, E
dc.contributor.authorTapia, AA
dc.date.accessioned2025-01-21T01:08:29Z
dc.date.available2025-01-21T01:08:29Z
dc.date.issued2004
dc.description.abstractA rapid in vitro propagation system leading to the formation of shoots, calli, roots, cell suspensions and plantlets was developed for the Andean medicinal plant Fabiana imbricata (Solanaceae). Massive propagation of shoots and roots was achieved by the temporary immersion system (TIS), morphogenesis and maintenance of cell suspensions by standard in vitro culture techniques. Oleanolic acid (OA), rutin, chlorogenic acid (CA) and scopoletin content in aerial parts of wild growing Fabiana imbricata plants as well as in plantlets regenerated in vitro, callus cultures, cell suspensions and biomass, obtained by the TIS system was assessed by HPLC.
dc.description.abstractOn a dry weight basis, the OA content in the aerial parts of the plant ranged between 2.26 and 3.47% while in vitro plantlets, callus and root cultures presented values ranging from not detected up to 0.14%. The rutin content of the samples presented a similar trend with maxima between 0.99 and 3.35% for the aerial parts of the plants to 0.02 to 0.20% for plantlets, 0.12% for cell suspensions and 0.28% for callus. Rutin was not detected in the roots grown by the TIS principle. The CA and scopoletin content in the aerial parts of F imbricata ranged between 0.22-1.15 and < 0.01-0.55%, respectively. In the plantlets, the concentration of CA was 0.29 to 1.48% with scopoletin in the range 0.09 to 0.64% while in the callus sample, the CA and scopoletin content were 0.46 and 0.66%, respectively. A very different result was found in roots grown by TIS, where both OA and rutin were not detected and its main secondary metabolite, scopoletin was found between a range of 0.99 and 1.41% with CA between of 0.11 and 0.42%.
dc.fuente.origenWOS
dc.identifier.eissn1865-7125
dc.identifier.issn0939-5075
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96474
dc.identifier.wosidWOS:000220790400011
dc.issue.numero1-2
dc.language.isoen
dc.pagina.final54
dc.pagina.inicio48
dc.revistaZeitschrift fur naturforschung section c-a journal of biosciences
dc.rightsacceso restringido
dc.subjectFabiana inibricata
dc.subjectin vitro propagation
dc.subjectsecondary metabolite content
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleSecondary metabolite content in <i>Fabiana imbricata</i> plants and <i>in vitro</i> cultures
dc.typeartículo
dc.volumen59
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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