Direct and indirect in vitro organogensis of Leucocoryne purpurea (Alliaceae) a chilean ornamental geophyte

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dc.contributor.authorEscobar, Luis H.
dc.contributor.authorJordan, Miguel
dc.contributor.authorOlate, Eduardo
dc.contributor.authorBarrales, Luis
dc.contributor.authorGebauer, Marlene
dc.date.accessioned2024-01-10T12:04:56Z
dc.date.available2024-01-10T12:04:56Z
dc.date.issued2008
dc.description.abstractIn vitro shoot induction from callus and direct bulbil formation from shoots were obtained in Leucocoryne purpurea, an endemic vulnerable Chilean species. Basal leaf portions, bulb basal plate and root tips from in vitro gown plants were used as explants. Only bulb basal plate explants displayed morphogenic responses. Callus, shoot and bulbils developed on MS basal medium in the presence of various growth regulator combinations. Callus formation from bulb basal explants ranged between 40 to 100%; the highest response was observed in the presence of 4.5 or 13.6 mu M 2,4-D alone or 4.5 mu M 2,4-D in combination with 0.5 mu M BA after 4 months. Picloram alone or in combination with 2iP or BAP also supported callus initiation. Indirect shoot formation from callus occurred only after a further culture period of 2 months in MS medium containing 0.5 or 2.3 mu M TDZ. Basal bulb plate explants cultured in the presence of TDZ alone (0.5 and 2.3 RM, respectively) exhibited direct shoot formation within 90 days. After subculture in the presence of 0.5 mu M TDZ each single shoot formed new shoots (3 to 4) and new bulbils. However higher TDZ levels, up to 2.3 mu M, produced shoots with roots only, inhibiting bulbil formation, and the 4.5 mu M TDZ concentration caused necrosis.
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.issn1311-9109
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75914
dc.identifier.wosidWOS:000255472500002
dc.information.autorucAgronomía e Ing. Forestal;Barrales L;S/I;99110
dc.information.autorucAgronomía e Ing. Forestal;Escobar L;S/I;66279
dc.information.autorucAgronomía e Ing. Forestal;Gebauer M;S/I;100191
dc.information.autorucCiencias Biológicas;Jordan M;S/I;98759
dc.information.autorucAgronomía e Ing. Forestal;Olate E;S/I;86197
dc.issue.numero2
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final64
dc.pagina.inicio59
dc.publisherSEJANI PUBL
dc.revistaPROPAGATION OF ORNAMENTAL PLANTS
dc.rightsregistro bibliográfico
dc.subjectbulbil formation
dc.subjectcallus induction
dc.subjectdirect and indirect organogenesis
dc.subjectLeucocoryne purpurea
dc.subjectmicropropagation
dc.subjectshoot formation
dc.subjectPLANT-REGENERATION
dc.subjectHIGH-FREQUENCY
dc.subjectCALLUS
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleDirect and indirect in vitro organogensis of Leucocoryne purpurea (Alliaceae) a chilean ornamental geophyte
dc.typeartículo
dc.volumen8
sipa.codpersvinculados99110
sipa.codpersvinculados66279
sipa.codpersvinculados100191
sipa.codpersvinculados98759
sipa.codpersvinculados86197
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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