Conformational Changes of Poly(Maleic Anhydride-<i>alt</i>-styrene) Modified with Amino Acids in an Aqueous Medium and Their Effect on Cytocompatibility and Hemolytic Response

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dc.contributor.authorMaine, Arianne
dc.contributor.authorTamayo, Laura
dc.contributor.authorLeiva, Angel
dc.contributor.authorGonzalez, Alex
dc.contributor.authorRios, Hernan E.
dc.contributor.authorRojas-Romo, Carlos
dc.contributor.authorJara, Paul
dc.contributor.authorAraya-Duran, Ingrid
dc.contributor.authorGonzalez-Nilo, Fernando
dc.contributor.authorYazdani-Pedram, Mehrdad
dc.contributor.authorSantana, Paula
dc.contributor.authorLeal, Matias
dc.contributor.authorGonzalez, Nicolas
dc.contributor.authorBriones, Ximena
dc.contributor.authorVillalobos, Valeria
dc.contributor.authorUrzua, Marcela
dc.date.accessioned2025-01-20T17:21:08Z
dc.date.available2025-01-20T17:21:08Z
dc.date.issued2023
dc.description.abstractThe conformational changes of poly-(maleic anhydride-alt-styrene) (PSMA) modified with different amino acids (PSMA-Aa) were studied in an aqueous medium as a function of ionic strength and pH. The specific viscosity of PSMA-Aa decreased with increasing salt concentration due to a more compact conformation. There was a decrease in surface tension with increasing concentrations of the modified polyelectrolyte having a greater effect for the PSMA modified with l-phenylalanine at pH 7.0, demonstrating a greater surface-active character. The conformational changes were also confirmed by molecular dynamics studies, indicating that PSMA-Aa exhibits a compact structure at pH 4.0 and a more extended structure at pH 7.0. On the other hand, the conformational changes of PSMA-Aa were related to its biological response, where the higher surface-active character of the PSMA modified with l-phenylalanine correlates very well with the higher hemolytic activity observed in red blood cells, in which the surface-active capacity supports lytic potency in erythrocytes. The cytocompatibility assays indicated that there were no significant cytotoxic effects of the PSMA-Aa. Additionally, in solvent-accessible surface area studies, it was shown that the carboxylate groups of the PSMA modified with l-phenylalanine are more exposed to the solvent at pH 7.0 and high salt concentrations, which correlates with lower fluorescence intensity, reflecting a loss of mitochondrial membrane potential. It is concluded that the study of the conformational changes in PE modified with amino acids is essential for their use as biomaterials and relevant to understanding the possible effects of PE modified with amino acids in biological systems.
dc.fuente.origenWOS
dc.identifier.doi10.1021/acsabm.3c00603
dc.identifier.issn2576-6422
dc.identifier.urihttps://doi.org/10.1021/acsabm.3c00603
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91447
dc.identifier.wosidWOS:001127536200001
dc.issue.numero12
dc.language.isoen
dc.pagina.final5348
dc.pagina.inicio5333
dc.revistaAcs applied bio materials
dc.rightsacceso restringido
dc.subjectmaleic anhydride
dc.subjectamino acid
dc.subjectcytocompatibility
dc.subjecthemolytic activity
dc.subjectpolyelectrolytes
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleConformational Changes of Poly(Maleic Anhydride-<i>alt</i>-styrene) Modified with Amino Acids in an Aqueous Medium and Their Effect on Cytocompatibility and Hemolytic Response
dc.typeartículo
dc.volumen6
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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