Ileal bile acid transport regulates bile acid pool, synthesis, and plasma cholesterol levels differently in cholesterol-fed rats and rabbits

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dc.contributor.authorXu, GR
dc.contributor.authorShneider, BL
dc.contributor.authorShefer, S
dc.contributor.authorNguyen, LB
dc.contributor.authorBatta, AK
dc.contributor.authorTint, GS
dc.contributor.authorArrese, M
dc.contributor.authorThevananther, S
dc.contributor.authorMa, L
dc.contributor.authorStengelin, S
dc.contributor.authorKramer, W
dc.contributor.authorGreenblatt, D
dc.contributor.authorPcolinsky, M
dc.contributor.authorSalen, G
dc.date.accessioned2024-01-10T12:39:35Z
dc.date.available2024-01-10T12:39:35Z
dc.date.issued2000
dc.description.abstractWe investigated the effect of heal bile acid transport on the regulation of classic and alternative bile acid synthesis in cholesterol-fed rats and rabbits. Bile acid pool sizes, fecal bile acid outputs (synthesis rates), and the activities of cholesterol 7 alpha-hydroxylase (classic bile acid synthesis) and cholesterol 27-hydroxylase (alternative bile acid synthesis) were related to heal bile acid transporter expression (ileal apical sodium-dependent bile acid transporter, ASBT). Plasma cholesterol levels rose 2.1-times in rats (98 +/- 19 mg/dl) and 31-times (986 +/- 188 mg/dl) in rabbits. The bile acid pool size remained constant (55 +/- 17 mg vs. 61 +/- 18 mg) in rats but doubled (254 +/- 46 to 533 +/- 53 mg) in rabbits. ASBT protein expression did not change in rats but rose 31% (P < 0.05) in rabbits, Fecal bile acid outputs that reflected bile acid synthesis increased 2- and 2.4-times (P < 0.05) in cholesterol-fed rats and rabbits, respectively. Cholesterol 7 alpha-hydroxylase activity rose 33% (24 +/- 2.4 vs. 18 +/- 1.6 pmol/mg/min, P < 0.01) and mRNA levels increased 50% (P < 0.01) in rats but decreased 68% and 79%, respectively, in cholesterol-fed rabbits. Cholesterol 27-hydroxylase activity remained unchanged in rats but rose 62% (P < 0.05) in rabbits. Classic bile acid synthesis (cholesterol 7 alpha-hydroxylase) was inhibited in rabbits because an enlarged bile acid pool developed from enhanced ileal bile acid transport. In contrast, in rats, cholesterol 7 alpha-hydroxylase was stimulated but the bile acid pool did not enlarge because ASBT did not change. jlr Therefore, although bile acid synthesis was increased via different pathways in rats and rabbits, enhanced ileal bile acid transport was critical for enlarging the bile acid pool size that exerted feedback regulation on cholesterol 7 alpha-hydroxylase in rabbits.
dc.description.funderNATIONAL HEART, LUNG, AND BLOOD INSTITUTE
dc.description.funderNATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
dc.description.funderNHLBI NIH HHS
dc.description.funderNIDDK NIH HHS
dc.fechaingreso.objetodigital2024-06-25
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.issn0022-2275
dc.identifier.pubmedidMEDLINE:10681414
dc.identifier.urihttps://doi.org/10.1016/S0022-2275(20)32064-2
dc.identifier.wosidWOS:000085510200016
dc.information.autorucMedicina;Arrese M;S/I;76095
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final304
dc.pagina.inicio298
dc.publisherLIPID RESEARCH INC
dc.revistaJOURNAL OF LIPID RESEARCH
dc.rightsacceso abierto
dc.subjectbile acids
dc.subjectabsorption
dc.subjectbiosynthesis
dc.subjectcholesterol 7 alpha-hydroxylase
dc.subjectcholesterol 27-hydroxylase
dc.subjectHERITABLE HYPERLIPIDEMIC RABBITS
dc.subjectMESSENGER-RNA LEVELS
dc.subjectHMG-COA REDUCTASE
dc.subjectDIETARY-CHOLESTEROL
dc.subject7-ALPHA-HYDROXYLASE EXPRESSION
dc.subjectFEEDBACK-REGULATION
dc.subjectPROTEIN
dc.subjectILEUM
dc.subjectBINDING
dc.subjectHYPERCHOLESTEROLEMIA
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIleal bile acid transport regulates bile acid pool, synthesis, and plasma cholesterol levels differently in cholesterol-fed rats and rabbits
dc.typeartículo
dc.volumen41
sipa.codpersvinculados76095
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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