Increased aortic NADPH oxidase activity in rats with genetically high angiotensin-converting enzyme levels

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dc.contributor.authorJalil, JE
dc.contributor.authorPerez, A
dc.contributor.authorOcaranza, MP
dc.contributor.authorBargetto, J
dc.contributor.authorGalaz, A
dc.contributor.authorLavandero, S
dc.date.accessioned2024-01-10T12:06:00Z
dc.date.available2024-01-10T12:06:00Z
dc.date.issued2005
dc.description.abstractIn humans and rats, angiotensin I-converting enzyme activity is significantly determined by a gene polymorphism. Homozygous Brown Norway rats have higher plasma angiotensin I-converting enzyme activity and circulating angiotensin II (Ang II) levels than Lewis rats. Because Ang II induces NAD(P) H oxidase activation, we hypothesized here that Brown Norway rats have higher vascular NAD(P) H oxidase activity and superoxide anion production than Lewis rats. Homozygous Brown Norway (n = 15) and Lewis (n = 13) male rats were used. Plasma angiotensin I-converting enzyme activity (by fluorimetry), Ang II levels (by high-performance liquid chromatography and radioimmunoassay), and aortic NAD(P) H oxidase activity, as well as superoxide anion production ( by chemiluminescence with lucigenin) were measured. Plasma angiotensin I-converting enzyme activity and Ang II levels were 100% higher in Brown Norway rats than in Lewis rats (P < 0.05). Aortic angiotensin I-converting enzyme, but not Ang II, was elevated (P < 0.05). Aortic superoxide anion production and NAD(P) H oxidase activity were 300% and 260% higher in Brown Norway than in Lewis rats, respectively (P < 0.05), which was not observed in Brown Norway rats treated with candesartan (10 mg/kg per day for 7 days). Endothelial NO synthase activity in the aorta from Brown Norway rats was significantly lower than in Lewis rats. However, inducible NO synthase activity and both endothelial NO synthase and inducible NO synthase mRNA and protein levels were similar in both genotypes. In summary, Brown Norway rats have higher vascular NAD(P) H oxidase activity and superoxide anion production than Lewis rats, suggesting the presence of a higher level of vascular oxidative stress in rats with genetically higher angiotensin I-converting enzyme levels. This effect is mediated through the angiotensin I receptor.
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1161/01.HYP.0000188980.57312.63
dc.identifier.eissn1524-4563
dc.identifier.issn0194-911X
dc.identifier.pubmedidMEDLINE:16230508
dc.identifier.urihttps://doi.org/10.1161/01.HYP.0000188980.57312.63
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76099
dc.identifier.wosidWOS:000233557700025
dc.information.autorucMedicina;Jalil J;S/I;99946
dc.information.autorucMedicina;Ocaranza M;S/I;1001254
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1367
dc.pagina.inicio1362
dc.publisherLIPPINCOTT WILLIAMS & WILKINS
dc.revistaHYPERTENSION
dc.rightsacceso restringido
dc.subjectangiotensin-converting enzyme
dc.subjectpolymorphism
dc.subjectnitric oxide
dc.subjectSMOOTH-MUSCLE-CELLS
dc.subjectINSERTION DELETION POLYMORPHISM
dc.subjectSUPEROXIDE-PRODUCTION
dc.subjectPHOSPHOLIPASE-D
dc.subjectBLOOD-PRESSURE
dc.subjectNEUTRAL ENDOPEPTIDASE
dc.subjectNADH/NADPH OXIDASE
dc.subjectGENE POLYMORPHISM
dc.subjectHYPERTENSIVE-RATS
dc.subjectNAD(P)H OXIDASE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIncreased aortic NADPH oxidase activity in rats with genetically high angiotensin-converting enzyme levels
dc.typeartículo
dc.volumen46
sipa.codpersvinculados99946
sipa.codpersvinculados1001254
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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