Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles

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dc.contributor.authorSanchez, G
dc.contributor.authorCuellar, D
dc.contributor.authorZulantay, I
dc.contributor.authorGajardo, M
dc.contributor.authorGonzalez Martin, G
dc.date.accessioned2024-01-10T12:04:48Z
dc.date.available2024-01-10T12:04:48Z
dc.date.issued2002
dc.description.abstractThe aim of the present study was to study the trypanocidal activity of nanoparticles loaded with nifurtimox in comparison with the free drug against Trypanosoma cruzi, responsible for Chagas' disease. Ethylcyanoacrylate nanoparticles acted as the delivery system into cells. As the obligate replicative intracellular form is amastigote, in vitro studies were performed on this form of parasite as well as on cell culture derived trypomastigotes. The fluorescence method used here was very useful as it allowed for the simultaneous study of trypanocide activity and cytotoxicity by determining living or dead parasites within living or dead host cells. According to these results, the greatest trypanocide activity on cell culture-derived trypomastigotes was recorded for nifurtimox-loaded nanoparticles with a 50% inhibitory concentration (IC50) twenty times less than that of the free drug. The cytotoxycity of unloaded nanoparticles at low concentrations was similar to that obtained by free drug when evaluated on Vero cells. Furthermore, nifurtimox-loaded nanoparticles showed increased trypanocide activity on intracellular amastigotes with an IC50 thirteen times less than that of nifurtimox. We also observed that the unloaded nanoparticles possess the previously-described trypanocide activity, similar to the standard solution of nifurtimox, although the mechanism for this has not yet been elucidated. In conclusion, it was possible to establish in vitro conditions using nifurtimox encapsulated nanoparticles in order to decrease the doses of the drug and thus to obtain high trypanocidal activity on both free trypomastigotes and intracellular amastigotes with low cytotoxicity for the host cell.
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.issn0716-9760
dc.identifier.pubmedidMEDLINE:12125203
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75885
dc.identifier.wosidWOS:000176107700006
dc.information.autorucQuímica;González G;S/I;98524
dc.issue.numero1
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final45
dc.pagina.inicio39
dc.publisherSOCIEDAD BIOLGIA CHILE
dc.revistaBIOLOGICAL RESEARCH
dc.rightsregistro bibliográfico
dc.subjectnifurtimox
dc.subjectnanoparticles
dc.subjectamastigotes
dc.subjecttrypomastigotes
dc.subjectTrypanosoma cruzi
dc.subjectTRYPANOSOMA-CRUZI
dc.subjectDRUG CARRIER
dc.subjectPARASITEMIA
dc.subjectMICE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
dc.typeartículo
dc.volumen35
sipa.codpersvinculados98524
sipa.indexWOS
sipa.indexScielo
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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