A multispecies outbreak of carbapenem-resistant bacteria harboring the blaKPC gene in a non-classical transposon element

Simple item page
dc.contributor.authorWozniak Banchero, Aniela
dc.contributor.authorFigueroa, Cristian
dc.contributor.authorMoya-Flores, Francisco
dc.contributor.authorGuggiana, Piero
dc.contributor.authorCastillo, Claudia
dc.contributor.authorRivas Jiménez, Lina María
dc.contributor.authorMunita, José M.
dc.contributor.authorGarcía Cañete, Patricia
dc.date.accessioned2021-04-13T11:36:30Z
dc.date.available2021-04-13T11:36:30Z
dc.date.issued2021
dc.date.updated2021-04-11T00:02:12Z
dc.description.abstractAbstract Background Klebsiella pneumoniae is the most frequent KPC-producing bacteria. The blaKPC gene is frequently embedded in Tn4401 transposon, and less frequently in non-Tn4401 elements (NTEKPC) variants I-III. The first case of KPC in the UC-CHRISTUS Clinical Hospital was detected in Pseudomonas aeruginosa. Soon after this event, KPC was detected in 2 additional Pseudomonas aeruginosa, 3 Escherichia coli, 3 Enterobacter cloacae, 3 Klebsiella pneumoniae, and 1 Citrobacter freundii, isolated from 6 different patients. We aimed to elucidate the possible mechanisms of genetic transfer and dissemination of the blaKPC gene among isolates of this multispecies outbreak. A molecular epidemiology analysis of the above mentioned clinical isolates (n = 13) through Multi-Locus Sequence Typing, plasmid analysis, Pulsed-Field Gel-Electrophoresis, and Whole-genome sequencing (WGS) was performed. Results High-risk sequence types were found: K. pneumoniae ST11, P. aeruginosa ST654, and E. cloacae ST114. All enterobacterial isolates were not clonal except for 3 E. coli isolated from the same patient. WGS analysis in 6 enterobacterial isolates showed that 4 of them had blaKPC embedded in a novel variant of NTEKPC designated NTEKPC-IIe. Upstream of blaKPC gene there was a 570 pb truncated blaTEM-1 gene followed by an insertion sequence that was 84% similar to ISEc63, a 4473 bp element of the Tn3 family. Downstream the blaKPC gene there was a truncated ISKpn6 gene, and the inverted repeat right sequence of Tn4401. The ISec63-like element together with the blaKPC gene plus Tn4401 remnants were inserted in the Tra operon involved in conjugative transfer of the plasmid. This NTE was carried in a broad host-range IncN plasmid. P. aeruginosa isolates carried blaKPC gene embedded in a typical Tn4401b transposon in a different plasmid, suggesting that there was no plasmid transfer between Enterobacteriaceae and P. aeruginosa as initially hypothesized. Conclusions Most enterobacterial isolates had blaKPC embedded in the same NTEKPC-IIe element, suggesting that this multispecies KPC outbreak was due to horizontal gene transfer rather than clonal spread. This poses a greater challenge to infection control measures often directed against containment of clonal spread.
dc.format.extent10 páginas
dc.identifier.citationBMC Microbiology. 2021 Apr 09;21(1):107
dc.identifier.doi10.1186/s12866-021-02169-3
dc.identifier.urihttps://doi.org/10.1186/s12866-021-02169-3
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/57633
dc.issue.numeroNo. 107
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final10
dc.pagina.inicio1
dc.revistaBMC Microbiologyes_ES
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subjectKPC Carbapenemasees_ES
dc.subjectCarbapenem resistant Enterobacteriaceaees_ES
dc.subjectMultispecies outbreakes_ES
dc.subjectHorizontal gene transferes_ES
dc.subject.ddc616.2412
dc.subject.deweyMedicina y saludes_ES
dc.titleA multispecies outbreak of carbapenem-resistant bacteria harboring the blaKPC gene in a non-classical transposon elementes_ES
dc.typeartículo
dc.volumenVol. 21
sipa.codpersvinculados1008612
sipa.codpersvinculados73909
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12866_2021_Article_2169.pdf
Size:
1.16 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos de revistas