Performance of the VITEK MS System for the Identification of Filamentous Fungi in a Routine Microbiological Laboratory in Chile

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dc.catalogadorgjm
dc.contributor.authorPorte, Lorena
dc.contributor.authorCruz, Rodrigo
dc.contributor.authorPérez, Inia
dc.contributor.authorVarela, Carmen
dc.contributor.authorDíaz, Cristina
dc.contributor.authorGarcía Cañete, Patricia
dc.contributor.authorLegarraga, Paulette
dc.contributor.authorValdivieso, Francisca
dc.contributor.authorWeitzel, Thomas
dc.date.accessioned2024-08-22T20:28:25Z
dc.date.available2024-08-22T20:28:25Z
dc.date.issued2024
dc.description.abstractBackground: Filamentous fungi are an emergent cause of severe infections in immunocompromised patients. Timely and accurate identification is crucial to initiate appropriate therapy. Traditional identification methods are time-consuming, labor-intensive, and operator-dependent. Ma-trix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry is a rapid and easy-to-perform identification method. This study evaluates the effectiveness of a commercial MALDI-TOF MS platform to identify filamentous fungi in a routine laboratory. Material and Methods: We included 67 fungal isolates from 35 species/species complexes within 15 genera, confirmed in mycology reference laboratories. 33 were from clinical samples and 34 from strain collections. The study used the VITEK MS system (v3.2.0 database), after sample extraction by VITEK MS Mould Kit. Results were classified into categories: ‘correct species’, ‘correct species complex’, ‘correct genus’, ‘incorrect identification’, and ‘no identification’. We also evaluated the practicality of the kit. Results: VITEK MS correctly identified 91.0% of isolates (58.2% to species, 29.9% to species complex, and 1.5% to genus level). In 82%, the result matched the species/species complex identified by reference methods. No misidentifications were observed. The kit was rapid and easy to use. Conclusion: The VITEK MS system showed a high capability to accurately identify filamentous fungi in a routine laboratory.
dc.fechaingreso.objetodigital2024-08-22
dc.format.extent10 páginas
dc.fuente.origenORCID
dc.identifier.doi10.20944/preprints202408.0457.v1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/87629
dc.information.autorucEscuela de Medicina; García Cañete, Patricia; 0000-0002-3817-4896; 73909
dc.language.isoen
dc.nota.accesocontenido completo
dc.rightsacceso abierto
dc.rights.licenseCC BY 4.0 Attribution 4.0 International Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDiagnosis
dc.subjectMALDI-TOF
dc.subjectFungal infections
dc.subjectMycology
dc.subjectMass spectrometry
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePerformance of the VITEK MS System for the Identification of Filamentous Fungi in a Routine Microbiological Laboratory in Chile
dc.typepreprint
sipa.codpersvinculados73909
sipa.trazabilidadORCID;2024-08-19
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