Cardiorespiratory alterations following intermittent photostimulation of RVLM C1 neurons: Implications for long-term blood pressure, breathing and sleep regulation in freely moving rats

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dc.contributor.authorToledo, Camilo
dc.contributor.authorAndrade, David C.
dc.contributor.authorDiaz-Jara, Esteban
dc.contributor.authorOrtolani, Domiziana
dc.contributor.authorBernal-Santander, Ignacio
dc.contributor.authorSchwarz, Karla G.
dc.contributor.authorOrtiz, Fernando C.
dc.contributor.authorMarcus, Noah J.
dc.contributor.authorOliveira, Luiz M.
dc.contributor.authorTakakura, Ana C.
dc.contributor.authorMoreira, Thiago S.
dc.contributor.authorDel Rio, Rodrigo
dc.date.accessioned2025-01-20T21:03:14Z
dc.date.available2025-01-20T21:03:14Z
dc.date.issued2022
dc.description.abstractAim Sympathoexcitation and sleep-disordered breathing are common contributors for disease progression. Catecholaminergic neurons from the rostral ventrolateral medulla (RVLM-C1) modulate sympathetic outflow and have anatomical projections to respiratory neurons; however, the contribution of highly selective activation of RVLM-C1 neurons on long-term autonomic and breathing (dys)regulation remains to be understood. Methods To explore this relationship, a lentiviral vector carrying the light-sensitive cation channel channelrhodopsin-2 (LVV-PRSX8-ChR2-YFP) was unilaterally injected into the RVLM of healthy rats. On the contralateral side, LVV-PRSX8-ChR2-YFP was co-injected with a specific immunotoxin (D beta H-SAP) targeted to eliminate C1 neurons. Results Intermittent photostimulation of RVLM-C1 in vivo, in unrestrained freely moving rats, elicited long-term facilitation of the sympathetic drive, a rise in blood pressure and sympatho-respiratory coupling. In addition, photoactivation of RVLM-C1 induced long-lasting ventilatory instability, characterized by oscillations in tidal volume and increased breathing variability, but only during non-rapid eye movement sleep. These effects were not observed when photostimulation of the RVLM was performed in the presence of D beta H-SAP toxin. Conclusions The finding that intermittent activation of RVLM-C1 neurons induces autonomic and breathing dysfunction suggest that episodic stimulation of RVLM-C1 may serve as a pathological substrate for the long-term development of cardiorespiratory disorders.
dc.fuente.origenWOS
dc.identifier.doi10.1111/apha.13864
dc.identifier.eissn1748-1716
dc.identifier.issn1748-1708
dc.identifier.urihttps://doi.org/10.1111/apha.13864
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93127
dc.identifier.wosidWOS:000842801100001
dc.issue.numero3
dc.language.isoen
dc.revistaActa physiologica
dc.rightsacceso restringido
dc.subjectbrainstem sympathetic neurons
dc.subjectbreathing irregularity
dc.subjectcardiorespiratory control
dc.subjectRVLM-C1 neurons
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCardiorespiratory alterations following intermittent photostimulation of RVLM C1 neurons: Implications for long-term blood pressure, breathing and sleep regulation in freely moving rats
dc.typeartículo
dc.volumen236
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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