Glucose increases intracellular free Ca2+ in tanycytes via ATP released through connexin 43 hemichannels

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dc.catalogadorpva
dc.contributor.authorOrellana Roca, Juan Andrés
dc.contributor.authorSáez Pedraza, Pablo José
dc.contributor.authorCortés-Campos, Christian
dc.contributor.authorElizondo, Roberto J.
dc.contributor.authorShoji Sánchez, Kenji Fabricio
dc.contributor.authorContreras Duarte, Susana de las Mercedes
dc.contributor.authorFigueroa, Vania
dc.contributor.authorVelarde Aliaga, María Victoria
dc.contributor.authorJiang, Jean X.
dc.contributor.authorNualart, Francisco
dc.contributor.authorSáez, Juan Carlos
dc.contributor.authorGarcía, María A.
dc.date.accessioned2024-10-14T19:09:18Z
dc.date.available2024-10-14T19:09:18Z
dc.date.issued2012
dc.description.abstractThe ventromedial hypothalamus is involved in regulating feeding and satiety behavior, and its neurons interact with specialized ependymal-glial cells, termed tanycytes. The latter express glucose-sensing proteins, including glucose transporter 2, glucokinase, and ATP-sensitive K+ (KATP) channels, suggesting their involvement in hypothalamic glucosensing. Here, the transduction mechanism involved in the glucose-induced rise of intracellular free Ca2+ concentration ([Ca2+]i) in cultured beta-tanycytes was examined. Fura-2AM time-lapse fluorescence images revealed that glucose increases the intracellular Ca2+ signal in a concentration-dependent manner. Glucose transportation, primarily via glucose transporters, and metabolism via anaerobic glycolysis increased connexin 43 (Cx43) hemichannel activity, evaluated by ethidium uptake and whole cell patch clamp recordings, through a KATP channel-dependent pathway. Consequently, ATP export to the extracellular milieu was enhanced, resulting in activation of purinergic P2Y1 receptors followed by inositol trisphosphate receptor activation and Ca2+ release from intracellular stores. The present study identifies the mechanism by which glucose increases [Ca2+]i in tanycytes. It also establishes that Cx43 hemichannels can be rapidly activated under physiological conditions by the sequential activation of glucosensing proteins in normal tanycytes
dc.fechaingreso.objetodigital2024-10-14
dc.fuente.origenConveris
dc.identifier.doi10.1002/glia.21246
dc.identifier.eissn1098-1136
dc.identifier.issn0894-1491
dc.identifier.pubmedidPMID 21987367
dc.identifier.urihttps://doi.org/10.1002/glia.21246
dc.identifier.urihttps://publons.com/wos-op/publon/10192696/
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/88201
dc.identifier.wosidWOS:000297213400005
dc.information.autorucFacultad de Ciencias Biológicas; Orellana Roca, Juan Andrés; 0000-0003-4076-207X; 126007
dc.information.autorucFacultad de Ciencias Biológicas; Sáez Pedraza, Pablo José; 0000-0003-0521-9426; 132607
dc.information.autorucFacultad de Ciencias Biológicas; Shoji Sánchez, Kenji Fabricio; S/I; 134136
dc.information.autorucFacultad de Ciencias Biológicas; Contreras Duarte, Susana de las Mercedes; 0000-0002-4198-7011; 149705
dc.information.autorucFacultad de Ciencias Biológicas; Velarde Aliaga, María Victoria; S/I; 55362
dc.information.autorucFacultad de Ciencias Biológicas; Sáez, Juan Carlos; 0000-0003-3811-0347; 99913
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final68
dc.pagina.inicio53
dc.publisherWiley Periodicals, Inc.
dc.revistaGLIA
dc.rightsacceso abierto
dc.subjectGlucosensing
dc.subjectHypothalamus
dc.subjectGlucokinase
dc.subjectConnexons
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGlucose increases intracellular free Ca2+ in tanycytes via ATP released through connexin 43 hemichannels
dc.typeartículo
dc.volumen60
sipa.codpersvinculados126007
sipa.codpersvinculados132607
sipa.codpersvinculados134136
sipa.codpersvinculados149705
sipa.codpersvinculados55362
sipa.codpersvinculados99913
sipa.indexISI
sipa.indexScopus
sipa.indexPubmed
sipa.indexWOS
sipa.trazabilidadConveris;20-07-2021
sipa.trazabilidadORCID;2024-10-14
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