Estetrol Increases Progesterone Genetic Response without Triggering Common Estrogenic Effects in Endometriotic Cell Lines and Primary Cultures

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dc.contributor.authorPatino-Garcia, Daniel
dc.contributor.authorPalomino, Jaime
dc.contributor.authorPomes, Cristian
dc.contributor.authorCelle, Claudia
dc.contributor.authorTorres-Estay, Veronica
dc.contributor.authorOrellana, Renan
dc.date.accessioned2025-01-20T20:14:59Z
dc.date.available2025-01-20T20:14:59Z
dc.date.issued2023
dc.description.abstractEstetrol (E4), a natural estrogen produced by the human fetal liver, is actively studied for menopause and breast cancer treatment. It has low side effects and preferential estrogen receptor alpha (ER alpha) affinity. There are no data about its effects on endometriosis, a common gynecological disease affecting 6-10% of cycling women, generating painful pelvic lesions and infertility. Current combined hormone treatment (progestins and estrogens) is safe and efficient; nevertheless, one-third of patients develop progesterone (P4) resistance and recurrence by reducing P4 receptors (PRs) levels. We aimed to compare E4 and 17 beta-estradiol (E2) effects using two human endometriotic cell lines (epithelial 11Z and stromal Hs832 cells) and primary cultures from endometriotic patients. We evaluated cell growth (MTS), migration (wound assay), hormone receptors levels (Western blot), and P4 response by PCR array. Compared to E2, E4 did not affect cell growth or migration but increased estrogen receptor alpha (ER alpha) and PRs, and reduced ER beta. Finally, the incubation with E4 improved the P4 gene response. In conclusion, E4 increased PRs levels and genetic response without inducing cell growth or migration. These results suggest that E4 might be useful for endometriosis treatment avoiding P4 resistance; however, evaluating its response in more complex models is required.
dc.fuente.origenWOS
dc.identifier.doi10.3390/biomedicines11041169
dc.identifier.eissn2227-9059
dc.identifier.urihttps://doi.org/10.3390/biomedicines11041169
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92233
dc.identifier.wosidWOS:000977351700001
dc.issue.numero4
dc.language.isoen
dc.revistaBiomedicines
dc.rightsacceso restringido
dc.subjectendometriosis
dc.subjectestetrol
dc.subjectprogesterone
dc.subjectresistance
dc.subjecthormones
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleEstetrol Increases Progesterone Genetic Response without Triggering Common Estrogenic Effects in Endometriotic Cell Lines and Primary Cultures
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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