Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract <i>in vitro</i>

Simple item page
dc.contributor.authorPaudel, Keshav Raj
dc.contributor.authorClarence, Dvya Delilaa
dc.contributor.authorPanth, Nisha
dc.contributor.authorManandhar, Bikash
dc.contributor.authorDe Rubis, Gabriele
dc.contributor.authorDevkota, Hari Prasad
dc.contributor.authorGupta, Gaurav
dc.contributor.authorZacconi, Flavia C.
dc.contributor.authorWilliams, Kylie A.
dc.contributor.authorPont, Lisa G.
dc.contributor.authorSingh, Sachin Kumar
dc.contributor.authorWarkiani, Majid Ebrahimi
dc.contributor.authorAdams, Jon
dc.contributor.authorMacloughlin, Ronan
dc.contributor.authorOliver, Brian G.
dc.contributor.authorChellappan, Dinesh Kumar
dc.contributor.authorHansbro, Philip Michael
dc.contributor.authorDua, Kamal
dc.date.accessioned2025-01-20T17:11:31Z
dc.date.available2025-01-20T17:11:31Z
dc.date.issued2024
dc.description.abstractThe purpose of this study was to evaluate the potential of zerumbone-loaded liquid crystalline nanoparticles (ZER-LCNs) in the protection of broncho-epithelial cells and alveolar macrophages against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro. The effect of the treatment of ZER-LCNs on in vitro cell models of cigarette smoke extract (CSE)-treated mouse RAW264.7 and human BCi-NS1.1 basal epithelial cell lines was evaluated for their anti-inflammatory, antioxidant and anti-senescence activities using colorimetric and fluorescence-based assays, fluorescence imaging, RT-qPCR and proteome profiler kit. The ZER-LCNs successfully reduced the expression of pro-inflammatory markers including Il-6, Il-1 beta and Tnf-alpha, as well as the production of nitric oxide in RAW 264.7 cells. Additionally, ZER-LCNs successfully inhibited oxidative stress through reduction of reactive oxygen species (ROS) levels and regulation of genes, namely GPX2 and GCLC in BCi-NS1.1 cells. Anti-senescence activity of ZER-LCNs was also observed in BCi-NS1.1 cells, with significant reductions in the expression of SIRT1, CDKN1A and CDKN2A. This study demonstrates strong in vitro anti-inflammatory, antioxidative and anti-senescence activities of ZER-LCNs paving the path for this formulation to be translated into a promising therapeutic agent for chronic respiratory inflammatory conditions including COPD and asthma.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00210-023-02760-7
dc.identifier.eissn1432-1912
dc.identifier.issn0028-1298
dc.identifier.urihttps://doi.org/10.1007/s00210-023-02760-7
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91188
dc.identifier.wosidWOS:001089629300003
dc.issue.numero4
dc.language.isoen
dc.pagina.final2483
dc.pagina.inicio2465
dc.revistaNaunyn-schmiedebergs archives of pharmacology
dc.rightsacceso restringido
dc.subjectZerumbone
dc.subjectLiquid crystalline nanoparticles
dc.subjectMonoolein
dc.subjectP407
dc.subjectAnti-inflammatory
dc.subjectAntioxidant
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleZerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract <i>in vitro</i>
dc.typeartículo
dc.volumen397
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Zerumbone liquid crystalline nanoparticles.pdf
Size:
4.14 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas