A new strategy to build electrochemical enzymatic biosensors using a nanohybrid material based on carbon nanotubes and a rationally designed schiff base containing boronic acid

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dc.contributor.authorTamborelli, Alejandro
dc.contributor.authorMujica, Michael Lopez
dc.contributor.authorSanchez-Velasco, Oriel A.
dc.contributor.authorHormazabal-Campos, Cristobal
dc.contributor.authorPerez, Edwin G.
dc.contributor.authorGutierrez-Cutino, Marlen
dc.contributor.authorVenegas-Yazigi, Diego
dc.contributor.authorDalmasso, Pablo
dc.contributor.authorRivas, Gustavo
dc.contributor.authorHermosilla-Ibanez, Patricio
dc.date.accessioned2025-01-20T17:09:38Z
dc.date.available2025-01-20T17:09:38Z
dc.date.issued2024
dc.description.abstractWe report a nanohybrid material obtained by non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with the new ligand (((1E,1 ' E)-(naphthalene-2,3-diylbis(azaneylylidene))bis(methaneylylidenedene)) bis (4-hyoxy-3,1-phenylene))doronic acid (SB-dBA), rationally designed to mimic some recognition properties of biomolecules like concanavalin A, for the development of electrochemical biosensors based on the use of glycobiomolecules as biorecognition element. We present, as a proof-of-concept, a hydrogen peroxide biosensor obtained by anchoring horseradish peroxidase (HRP) at a glassy carbon electrode (GCE) modified with the nanohybrid prepared by sonication of 2.0 mg mL- 1 MWCNTs and 0.50 mg mL- 1 SB-dBA in N,N-dimethyl formamide (DMF) for 30 min. The hydrogen peroxide biosensing was performed at -0.050 V in the presence of 5.0 x 10-4 M hydroquinone. The analytical characteristics of the resulting biosensor are the following: linear range between 0.175 mu M and 6.12 mu M, detection limit of 58 nM, and reproducibility of 2.0 % using the same nanohybrid (6 biosensors), and 9.0 % using three different nanohybrids. The sensor was successfully used to quantify hydrogen peroxide in enriched milk and human blood serum samples and in a commercial disinfector.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.talanta.2023.125520
dc.identifier.eissn1873-3573
dc.identifier.issn0039-9140
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2023.125520
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91059
dc.identifier.wosidWOS:001147515800001
dc.language.isoen
dc.revistaTalanta
dc.rightsacceso restringido
dc.subjectMulti-walled carbon nanotubes
dc.subjectBoronic acid
dc.subjectSchiff base
dc.subjectGlycoenzyme
dc.subjectHydrogen peroxide
dc.subjectElectrochemical biosensor
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA new strategy to build electrochemical enzymatic biosensors using a nanohybrid material based on carbon nanotubes and a rationally designed schiff base containing boronic acid
dc.typeartículo
dc.volumen270
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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