Abrogation of prostaglandin E<sub>2</sub>/EP4 signaling impairs the development of <i>rag1</i><SUP>+</SUP> lymphoid precursors in the thymus of zebrafish embryos

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dc.contributor.authorVillablanca, Eduardo J.
dc.contributor.authorPistocchi, Anna
dc.contributor.authorCourt, Felipe A.
dc.contributor.authorCotelli, Franco
dc.contributor.authorBordignon, Claudio
dc.contributor.authorAllende, Miguel L.
dc.contributor.authorTraversari, Catia
dc.contributor.authorRusso, Vincenzo
dc.date.accessioned2025-01-21T01:05:29Z
dc.date.available2025-01-21T01:05:29Z
dc.date.issued2007
dc.description.abstractPGE(2) is involved in a wide variety of physiological and pathological processes; however, deciphering its role in early Mammalian development has been difficult due to the maternal contribution of PGE(2). To overcome this limitation we have investigated the role of PGE(2) during T cell development in zebrafish. In this study, we show that zebrafish ep4a, a PGE(2) receptor isoform of EP4, is expressed at 26 h postfertilization in the dorsal aorta-posterior cardinal vein joint region, which has a high homology with the mammal aorta-gonad-mesonephros area and where definitive hemopoiesis arises. Furthermore, it is expressed in the presumptive thymus rudiment by 48 h postfertilization. Supplementation of PGE(2), results in a strong increase in rag 1 levels and cell proliferation in the thymus. In contrast, the inhibition of PGE(2) production, as well as EP4 blockade, abrogates the expression of rag1 in the thymus and that of the lymphoid precursor marker ikaros, not only in the dorsal aorta-posterior cardinal vein joint region but also in the newly identified caudal hemopoietic tissue without affecting early hemopoietic (scl, gata2) and erythropoietic (gatal1) markers. These results identify ep4a as the earliest thymus marker and define a novel role for the PGE(2)/EP4 pathway in controlling T cell precursor development in zebrafish.
dc.fuente.origenWOS
dc.identifier.issn0022-1767
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95934
dc.identifier.wosidWOS:000247497600045
dc.issue.numero1
dc.language.isoen
dc.pagina.final364
dc.pagina.inicio357
dc.revistaJournal of immunology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAbrogation of prostaglandin E<sub>2</sub>/EP4 signaling impairs the development of <i>rag1</i><SUP>+</SUP> lymphoid precursors in the thymus of zebrafish embryos
dc.typeartículo
dc.volumen179
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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