Platelet aging in vivo is associated with loss of membrane phospholipid asymmetry

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dc.contributor.authorPereira, J
dc.contributor.authorPalomo, I
dc.contributor.authorOcqueteau, M
dc.contributor.authorSoto, M
dc.contributor.authorAranda, E
dc.contributor.authorMezzano, D
dc.date.accessioned2024-01-10T12:41:31Z
dc.date.available2024-01-10T12:41:31Z
dc.date.issued1999
dc.description.abstractThe mechanism(s) involved in the clearance of senescent platelets are largely unknown. The loss of membrane phospholipid (PL) asymmetry, with phosphatidylserine (PS) exposure appears to be an important signal for the ingestion by macrophages of apoptotic nucleated cells and it has also been suggested as a signal for the removal of aged erythrocytes. Accordingly, it seems possible that the clearance of normal aged platelets from circulation might be triggered by PS exposure. To investigate this, we determined PS exposure in human aging platelets taking advantage of the relationship between platelet density and platelet age and in dog platelets in a model of platelet aging in vivo. PS exposure was determined in two experimental conditions: 1) human platelet density subpopulations obtained by centrifugation in arabinogalactan gradients; 2) circulating canine platelets during decline in platelet count after suppression of thrombopoiesis following estradiol injection. PS exposure was determined by flow cytometry after labeling the cells with FITC-conjugated annexin V. The proportion of human platelets with exposed PS was significantly higher in high density (HD) platelets compared to low density (LD) platelets (11.3 +/- 8.0% vs 5.2 +/- 3.7%; p < 0.05, respectively). In dogs, the proportion of cells with exposed PS rises dramatically with age, from 3.1 +/- 0.4% before to 17.7 +/- 12.3% ten days after estradiol injection. These findings suggest that platelet aging is associated with loss of phospholipid asymmetry and PS exposure on the outer leaflet of cell membrane, which may play an important role in the recognition and subsequent removal of senescent platelets.
dc.fechaingreso.objetodigital2024-06-14
dc.format.extent4 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1055/s-0037-1614383
dc.identifier.issn0340-6245
dc.identifier.pubmedidMEDLINE:10544921
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77425
dc.identifier.wosidWOS:000083145000023
dc.information.autorucMedicina;Mezzano D;S/I;99455
dc.information.autorucMedicina;Pereira J;S/I;99371
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1321
dc.pagina.inicio1318
dc.publisherF K SCHATTAUER VERLAG GMBH
dc.revistaTHROMBOSIS AND HAEMOSTASIS
dc.rightsacceso restringido
dc.subjectplatelet age
dc.subjectphosphatidylserine
dc.subjectplatelet removal
dc.subjectDENSITY SUBPOPULATIONS
dc.subjectBUOYANT DENSITY
dc.subjectBLOOD-CELLS
dc.subjectAPOPTOSIS
dc.subjectPHOSPHATIDYLSERINE
dc.subject5-HYDROXYTRYPTAMINE
dc.subjectPROTEIN
dc.subjectSIZE
dc.subjectAGE
dc.subjectACCUMULATION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePlatelet aging in vivo is associated with loss of membrane phospholipid asymmetry
dc.typeartículo
dc.volumen82
sipa.codpersvinculados99455
sipa.codpersvinculados99371
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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