Density and distribution of dendritiform cells in the peripheral cornea of healthy subjects using <i>in vivo</i> confocal microscopy

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dc.contributor.authorAkhlaq, Anam
dc.contributor.authorColon, Clara
dc.contributor.authorCavalcanti, Bernardo M.
dc.contributor.authorAggarwal, Shruti
dc.contributor.authorQazi, Yureeda
dc.contributor.authorCruzat, Andrea
dc.contributor.authorJersey, Candice
dc.contributor.authorCritser, Douglas B.
dc.contributor.authorWatts, Amy
dc.contributor.authorBeyer, Jill
dc.contributor.authorSindt, Christine W.
dc.contributor.authorHamrah, Pedram
dc.date.accessioned2025-01-20T21:01:45Z
dc.date.available2025-01-20T21:01:45Z
dc.date.issued2022
dc.description.abstractPurpose: To establish in a large healthy cohort, dendritiform cell (DC) density and morphological parameters in the central and peripheral cornea using in vivo confocal microscopy (IVCM).Methods: A prospective, cross-sectional, observational study was conducted in 85 healthy volunteers (n = 85 eyes). IVCM images of corneal center and four peripheral zones were analyzed for DC density and morphology to compare means and assess correlations (p < 0.05 being statistically significant).Results: Central corneas had lower DC density (40.83 +/- 5.14 cells/mm(2); mean +/- SEM) as compared to peripheral corneas (75.42 +/- 2.67 cells/mm(2), p < 0.0001). Inferior and superior zones demonstrated higher DC density (105.01 +/- 7.12 and 90.62 +/- 4.62 cells/mm(2)) compared to the nasal and temporal zones (59.93 +/- 3.42 and 51.77 +/- 2.98 cells/mm(2), p < 0.0001). Similarly, lower DC size, field and number of dendrites were observed in the central as compared to the average peripheral cornea (p < 0.0001), with highest values in the inferior zone (p < 0.001 for all, except p < 0.05 for number of dendrites in superior zone). DC parameters did not correlate with age or gender. Inter-observer reliability was 0.987 for DC density and 0.771-0.922 for morphology.Conclusion: In healthy individuals, the peripheral cornea demonstrates higher DC density and larger morphology compared to the center, with highest values in the inferior zone. We provide the largest normative cohort for sub -stratified DC density and morphology, which can be used in future clinical trials to compare differential changes in diseased states. Furthermore, as DC parameters in the peripheral zones are dissimilar, random sampling of peripheral cornea may be inaccurate.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.jtos.2022.07.008
dc.identifier.eissn1937-5913
dc.identifier.issn1542-0124
dc.identifier.urihttps://doi.org/10.1016/j.jtos.2022.07.008
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92948
dc.identifier.wosidWOS:000875045300004
dc.language.isoen
dc.pagina.final165
dc.pagina.inicio157
dc.revistaOcular surface
dc.rightsacceso restringido
dc.subjectCornea
dc.subjectCentral cornea
dc.subjectDendritic cells
dc.subjectIn vivo confocal microscopy
dc.subjectPeripheral cornea
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDensity and distribution of dendritiform cells in the peripheral cornea of healthy subjects using <i>in vivo</i> confocal microscopy
dc.typeartículo
dc.volumen26
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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