Adenosine 5′-triphosphate and neuropeptide Y are co-transmitters in conjunction with noradrenaline in the human saphenous vein

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dc.contributor.authorRacchi, H
dc.contributor.authorIrarrázabal, MJ
dc.contributor.authorHoward, M
dc.contributor.authorMorán, S
dc.contributor.authorZalaquett, R
dc.contributor.authorHuidobro-Toro, JP
dc.date.accessioned2025-01-21T01:32:09Z
dc.date.available2025-01-21T01:32:09Z
dc.date.issued1999
dc.description.abstract1 Human saphenous veins were used to assess the cooperative participation of adenosine 5-triphosphate (ATP), neuropeptide Y (NPY), and noradrenaline (NA) in the vasomotor responses elicited following electrical depolarization of the perivascular nerve terminals. Rings from recently dissected human biopsies were mounted to record isometric muscular contractions; the motor activity elicited in the circular muscle layer following electrical depolarization (2.5-20 Hz, 50 V, 0.5 msec) were recorded.
dc.description.abstract2 Incubation of the biopsies with either 100 nM tetrodotoxin (TTX) or 1 mu M guanethidine abolished the vasomotor response elicited by electrical nerve depolarization. The independent application of either ATP or NA to vein rings induced concentration-dependent contractions.
dc.description.abstract3 Tissue incubation with 30 mu M suramin or 10 nM prazosin produced 10 fold rightward displacements of the alpha,beta-methylene ATP and NA concentration-response curves respectively. NPY contracted a limited number of biopsies, the vasoconstriction elicited was completely blocked by 1 mu M BIBP 3226. A 5 min incubation of the biopsies with 10-100 nM NPY synergized, in a concentration-dependent fashion, both the ATP and the ATP analogue-induced contractions. Likewise, tissue preincubation with 10 nM NPY potentiated the vasomotor responses evoked with 20-60 nM NA.
dc.description.abstract4 Neither suramin, BIBP 3226, nor prazosin was individually able to significantly modify the derived frequency-tension curves. In contrast, the co-application of 30 mu M suramin and 10 nM prazosin or 30 mu M suramin and 1 mu M BIBP 3226, elicited a significant (P < 0.01) downward displacement of the respective frequency-tension curves.
dc.description.abstract5 The simultaneous application of the three antagonists-30 mu M suramin, 1 mu M BIBP 3226 and 10 nM prazosin-caused a significantly greater displacement of the frequency-tension curve than that achieved in experiments using two of these antagonists.
dc.description.abstract6 Electrically-evoked vasomotor activity is blocked to a larger extent by tissue incubation with 2.5 mu M chloroethylclonidine and 30 mu M suramin rather than with 10 nM 5 methyl urapidil and 30 mu M suramin. As a result, the alpha(1)-adrenoceptor involved in the vasomotor activity has tentatively been associated with the alpha(1B) adrenoceptor family subtype.
dc.description.abstract7 Results support the physiological role of ATP in sympathetic neurotransmission. The present results are consistent with the working hypothesis that human sympathetic vasomotor reflexes involve the coordinated motor action of ATP, NPY, and NA. acting on vascular smooth muscle cells. The present results support the concept of sympathetic co-transmission in the human saphenous vein.
dc.fuente.origenWOS
dc.identifier.eissn1476-5381
dc.identifier.issn0007-1188
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97214
dc.identifier.wosidWOS:000079052200014
dc.issue.numero5
dc.language.isoen
dc.pagina.final1185
dc.pagina.inicio1175
dc.revistaBritish journal of pharmacology
dc.rightsacceso restringido
dc.subjectco-transmission
dc.subjectsympathetic nerve activity
dc.subjectATP receptors
dc.subjectNPY vasomotor effect
dc.subjectnoradrenaline vasoconstriction
dc.subjecthuman saphenous vein
dc.subject.ods03 Good Health and Well-being
dc.subject.ods02 Zero Hunger
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa02 Hambre cero
dc.titleAdenosine 5′-triphosphate and neuropeptide Y are co-transmitters in conjunction with noradrenaline in the human saphenous vein
dc.typeartículo
dc.volumen126
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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