Genital sensory stimulation shifts estradiol intraoviductal signaling from nongenomic to genomic pathways, independently from prolactin surges

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dc.contributor.authorPenarroja Matutano, C.
dc.contributor.authorParada Bustamante, A.
dc.contributor.authorOrihuela, P. A.
dc.contributor.authorRios, M.
dc.contributor.authorVelasquez, Luis A.
dc.contributor.authorCroxatto, H. B.
dc.date.accessioned2024-01-10T13:13:18Z
dc.date.available2024-01-10T13:13:18Z
dc.date.issued2007
dc.description.abstractEstradiol (132) accelerates oviductal egg transport through nongenomic pathways involving oviductal protein phosphorylation in non-mated rats, and through genomic pathways in mated rats. Here we investigated the ability of cervico-vaginal stimulation (CVS) to switch the mode of action of E 2 in the absence of other male-associated components. Pro-estrous rats were subjected to CVS with a glass rod and 12 hours later were injected subcutaneously with E 2 and intrabursally with the RNA synthesis inhibitor Actinomycin D or the protein phosphorylation inhibitor H-89. The number of eggs in the oviduct, assessed 24 It later, showed that Actinomycin D, but not H-89 blocked the E-2-induced egg transport acceleration. This clearly indicates that CVS alone, without other mating-associated signals, is able to shift E 2 signaling from nongenomic to genomic pathways. Since mating and CVS activate a neuroendocrine reflex that causes iterative prolactin (PRL) surges, the involvement of PRL pathway in this phenomenon was evaluated. Prolactin receptor mRNA and protein expression in the rat oviduct was demonstrated by RT-PCR and Western blot, but their levels were not different on day 2 of the cycle (C2) or pregnancy (P2). Activated STAT 5a/b (phosphorylated) was detected by Western blot on P2 in the ovary, but not in the oviduct, showing that mating does not stimulate this PRL signalling pathway in the oviduct. Other rats subjected to CVS in the evening of pro-estrus were treated with bromoergocriptine to suppress PRL surges. In these rats, H-89 did not block the E-2-induced acceleration of egg transport suggesting that PRL surges are not essential to shift E-2 signaling pathways in the oviduct. We conclude that CVS is one of the components of mating that shifts E-2 signaling in the oviduct from nongenomic to genomic pathways, and this effect is independent of PRL surges elicited by mating.
dc.fechaingreso.objetodigital2024-06-25
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.pubmedidMEDLINE:18064358
dc.identifier.urihttp://dx.doi.org/10.4067/S0716-97602007000200012
dc.identifier.wosidWOS:000250812900012
dc.information.autorucMedicina;Croxatto H;S/I;98164
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final222
dc.pagina.inicio213
dc.publisherSOC BIOLGIA CHILE
dc.revistaBIOLOGICAL RESEARCH
dc.rightsacceso abierto
dc.subjectmating
dc.subjectoviduct
dc.subjectestradiol
dc.subjectsignaling pathways
dc.subjectprolactin receptor
dc.subjectegg transport
dc.subjectrat
dc.subjectTYROSINE KINASE
dc.subjectRECEPTOR GENE
dc.subjectDEVELOPMENTAL EXPRESSION
dc.subjectCORPUS-LUTEUM
dc.subjectRAT
dc.subjectTRANSPORT
dc.subjectPROTEIN
dc.subjectPHOSPHORYLATION
dc.subjectCELLS
dc.subjectACTIVATION
dc.subject.ods03 Good Health and Well-being
dc.subject.ods05 Gender Equality
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa05 Igualdad de género
dc.titleGenital sensory stimulation shifts estradiol intraoviductal signaling from nongenomic to genomic pathways, independently from prolactin surges
dc.typeartículo
dc.volumen40
sipa.codpersvinculados98164
sipa.indexWOS
sipa.indexScielo
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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