Salmonella enterica Serovar typhi 0 : 1,9,12 polysaccharide-protein conjugate as a diagnostic tool for typhoid fever

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dc.contributor.authorZuniga, J
dc.contributor.authorLillo, L
dc.contributor.authorShin, JJ
dc.contributor.authorMachavarapu, RL
dc.contributor.authorQuiroga, T
dc.contributor.authorGoycoolea, M
dc.contributor.authorMatsuhiro, B
dc.contributor.authorAron Hott, L
dc.contributor.authorGodfrey, HP
dc.contributor.authorCabello, FC
dc.date.accessioned2024-01-10T12:08:22Z
dc.date.available2024-01-10T12:08:22Z
dc.date.issued2005
dc.description.abstractSerologic tests play an important role in diagnosis of typhoid fever. In an effort to develop a more defined reagent for these tests, purified Salmonella enterica serovar Typhi (ST) 0:1,9,12 polysaccharide was conjugated to human serum albumin (HSA), and the conjugate was purified chromatographically to yield a reagent with 2 moles ST O polysaccharide per mole HSA. In 40 patients with bacteriologically confirmed typhoid fever, significant dot immunobinding titers (>= 20,000) were present in 28 (70%) tested with 100 ng of ST O antigen-HSA (ST O-HSA) conjugate, in 38 (95%) tested with 100 ng of ST lipopolysaccharide, and in 16 (40%) tested with purified unconjugated ST O chains. In sera from 22 patients with other nontyphoid fevers, 2 (9.1%) had such reactivities with 100 ng of ST O-HSA, 1 (4.5%) had such reactivity with 100 ng of ST lipopolysaccharide (4.5%), and none reacted with 100 ng of unconjugated ST O chains. None of the 17 healthy-control sera reacted significantly with any of the ST reagents. None of the patient or control sera reacted with unconjugated HSA. The sensitivity of dot immunobinding for typhoid fever was 70% with 100 ng of ST O-HSA, somewhat lower than that with 100 ng of ST lipopolysaccharide (95%) but similar to that of the Widal H agglutination test with a >= 1/160 cutoff (74%). Specificities of these tests were 91%, 95%, and 86%, respectively. These preliminary results suggest that ST O polysaccharide-protein conjugates could provide a nontoxic, easily quality-controlled synthetic reagent for analysis of human immune responses to ST as well as for the development of new diagnostics and vaccines for typhoid fever.
dc.description.funderNIAID NIH HHS
dc.description.funderNATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1128/JCM.43.9.4545-4550.2005
dc.identifier.eissn1098-660X
dc.identifier.issn0095-1137
dc.identifier.pubmedidMEDLINE:16145105
dc.identifier.urihttps://doi.org/10.1128/JCM.43.9.4545-4550.2005
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76385
dc.identifier.wosidWOS:000232020400036
dc.information.autorucMedicina;Quiroga S;S/I;52601
dc.issue.numero9
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final4550
dc.pagina.inicio4545
dc.publisherAMER SOC MICROBIOLOGY
dc.revistaJOURNAL OF CLINICAL MICROBIOLOGY
dc.rightsacceso restringido
dc.subjectLINKED-IMMUNOSORBENT-ASSAY
dc.subjectMONOCLONAL-ANTIBODIES
dc.subjectWIDAL TEST
dc.subjectLIPOPOLYSACCHARIDE
dc.subjectTUBERCULOSIS
dc.subjectSERODIAGNOSIS
dc.subjectANTIGENS
dc.subjectTESTS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSalmonella enterica Serovar typhi 0 : 1,9,12 polysaccharide-protein conjugate as a diagnostic tool for typhoid fever
dc.typeartículo
dc.volumen43
sipa.codpersvinculados52601
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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