Permeation of molecules through astroglial connexin 43 hemichannels is modulated by cytokines with parameters depending on the permeant species

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dc.article.number3970
dc.catalogadorgjm
dc.contributor.authorSáez, Juan Carlos
dc.contributor.authorVargas, Aníbal A.
dc.contributor.authorHernández, Diego E.
dc.contributor.authorOrtiz, Fernando C.
dc.contributor.authorGiaume, Christian
dc.contributor.authorOrellana Roca, Juan Andrés
dc.date.accessioned2024-10-16T16:23:30Z
dc.date.available2024-10-16T16:23:30Z
dc.date.issued2020
dc.description.abstractRecent studies indicate that connexin hemichannels do not act as freely permeable non-selective pores, but they select permeants in an isoform-specific manner with cooperative, competitive and saturable kinetics. The aim of this study was to investigate whether the treatment with a mixture of IL-1 beta plus TNF-alpha, a well-known pro-inflammatory condition that activates astroglial connexin 43 (Cx43) hemichannels, could alter their permeability to molecules. We found that IL-1 beta plus TNF-alpha left-shifted the dye uptake rate vs. dye concentration relationship for Etd and 2-NBDG, but the opposite took place for DAPI or YO-PRO-1, whereas no alterations were observed for Prd. The latter modifications were accompanied of changes in K-d (Etd, DAPI, YO-PRO-1 or 2-NBDG) and Hill coefficients (Etd and YO-PRO-1), but not in alterations of V-max. We speculate that IL-1 beta plus TNF-alpha may distinctively affect the binding sites to permeants in astroglial Cx43 hemichannels rather than their number in the cell surface. Alternatively, IL-1 beta plus TNF-alpha could induce the production of endogenous permeants that may favor or compete for in the pore-lining residues of Cx43 hemichannels. Future studies shall elucidate whether the differential ionic/molecule permeation of Cx43 hemichannels in astrocytes could impact their communication with neurons in the normal and inflamed nervous system.
dc.fechaingreso.objetodigital2024-10-16
dc.format.extent22 páginas
dc.fuente.origenORCID
dc.identifier.converisid1
dc.identifier.doi10.3390/ijms21113970
dc.identifier.issn1661-6596
dc.identifier.pubmedidMEDLINE:32492823
dc.identifier.scopusid2-s2.0-85085917669
dc.identifier.urihttps://doi.org/10.3390/ijms21113970
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/88265
dc.identifier.wosidWOS:000543400300232
dc.information.autorucEscuela de Medicina; Orellana Roca, Juan Andrés; 0000-0003-4076-207X; 126007
dc.information.autorucFacultad de Ciencias Biológicas; Sáez, Juan Carlos; 0000-0003-3811-0347; 99913
dc.issue.numero11
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaInternational Journal of Molecular Sciences
dc.rightsacceso abierto
dc.rights.licenseCC BY 4.0 Attribution 4.0 International Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePermeation of molecules through astroglial connexin 43 hemichannels is modulated by cytokines with parameters depending on the permeant species
dc.typeartículo
dc.volumen21
sipa.codpersvinculados126007
sipa.codpersvinculados99913
sipa.trazabilidadORCID;2024-10-14
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