Amino Acid-Functionalized Polyelectrolyte Films as Bioactive Surfaces for Cell Adhesion

Simple item page
dc.contributor.authorLeal, M. S.
dc.contributor.authorBriones, X.
dc.contributor.authorVillalobos, V.
dc.contributor.authorQueneau, Y.
dc.contributor.authorLeiva, A.
dc.contributor.authorRios, H. E.
dc.contributor.authorPavez, J.
dc.contributor.authorSilva, C. P.
dc.contributor.authorCarrasco, C.
dc.contributor.authorNeira-Carrillo, Andronico
dc.contributor.authorRoth, A. D.
dc.contributor.authorTamayo, L.
dc.contributor.authorUrzua, M. D.
dc.date.accessioned2025-01-23T21:12:54Z
dc.date.available2025-01-23T21:12:54Z
dc.date.issued2019
dc.description.abstractSurfaces were prepared with polyelectrolyte derivatives of poly(styrene-alt-maleic anhydride) (PSMA) functionalized with amino acids of different hydropathy indices, with the aim of evaluating the effect of the chemical functionality of polyelectrolytes on SH-SY5Y neuroblastoma cell adhesion. Functionalizing PSMA derivatives with L-glutamine, L-methionine, and L-tyrosine yielded PSMA-Gln, PSMA-Met, and PSMA-Tyr polyelectrolytes, respectively. We first studied the adsorption behavior of PSMA functionalized with amino acids on silicon wafer surfaces modified with 3-aminopropyltriethoxysilane at pH 4.0 and 7.0 and at low and high ionic strengths. The highest rate of polyelectrolyte adsorption was at pH 4.0 and high ionic strength and was higher with the glutamine and tyrosine films. The advance contact angles (BA) of the polyelectrolyte surfaces showed a moderate effect of ionic strength and pH on polyelectrolyte film wettability, with PSMA-Tyr being slightly more hydrophobic. Atomic force microscopy images of the polyelectrolyte surfaces showed two types of morphology: the well-defined globular nanostructure of PSMA-Met and PSMA-Tyr and densely packed nanofibrous-like structure of PSMA-Gln. The highest level of ionic strength caused a slight decrease in the size of the nanostructure that formed the surface domains, which was reflected in the degree of surface roughness. Cell adhesion assays with the polyelectrolyte film showed that SH-SY5Y neuroblastoma cells cultured on PSMA-Met present a well-extended morphology characterized by a stellate shape, with five or more actin-rich thin processes, whereas SH-SY5Y cells that were seeded on PSMA-Gln and PSMAT-yr have a round morphology, with fewer and shorter processes. These results indicate that it is possible to modulate the surface characteristics of polyelectrolyte films based on their chemical functionality and environmental parameters such as pH and ionic strength in order to evaluate their effect on cell adhesion. Thus, surfaces prepared from polyelectrolytes functionalized with amino acids are an attractive and simple platform for cell adhesion, which can be used in developing biomaterials with modulated surface properties.
dc.fuente.origenWOS
dc.identifier.doi10.1021/acsami.9b02503
dc.identifier.eissn1944-8252
dc.identifier.issn1944-8244
dc.identifier.urihttps://doi.org/10.1021/acsami.9b02503
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100982
dc.identifier.wosidWOS:000470938500009
dc.issue.numero22
dc.language.isoen
dc.pagina.final19762
dc.pagina.inicio19751
dc.revistaAcs applied materials & interfaces
dc.rightsacceso restringido
dc.subjectpolyelectrolytes
dc.subjectamino acid
dc.subjectfunctionalization
dc.subjectcell adhesion
dc.subjectSH-SY5Y neuroblastoma
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAmino Acid-Functionalized Polyelectrolyte Films as Bioactive Surfaces for Cell Adhesion
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas