Structural insights into a functional unit from an immunogenic mollusk hemocyanin

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dc.contributor.authorMunoz, Sebastian M.
dc.contributor.authorVallejos-Baccelliere, Gabriel
dc.contributor.authorManubens, Augusto
dc.contributor.authorSalazar, Michelle L.
dc.contributor.authorNascimento, Andrey F. Z.
dc.contributor.authorTapia-Reyes, Patricio
dc.contributor.authorMeneses, Claudio
dc.contributor.authorAmbrosio, Andre L. B.
dc.contributor.authorBecker, Maria Ines
dc.contributor.authorGuixe, Victoria
dc.contributor.authorCastro-Fernandez, Victor
dc.date.accessioned2025-01-20T16:14:04Z
dc.date.available2025-01-20T16:14:04Z
dc.date.issued2024
dc.description.abstractMollusk hemocyanins, among the largest known proteins, are used as immunostimulants in biomedical and clinical applications. The hemocyanin of the Chilean gastropod Concholepas concholepas (CCH) exhibits unique properties, which makes it safe and effective for human immunotherapy, as observed in animal models of bladder cancer and melanoma, and dendritical cell vaccine trials. Despite its potential, the structure and amino acid sequence of CCH remain unknown. This study reports two sequence fragments of CCH, representing three complete functional units (FUs). We also determined the high -resolution (1.5 A & ring; ) X-ray crystal structure of an "FU -g type"from the CCHB subunit. This structure enables in-depth analysis of chemical interactions at the copper -binding center and unveils an unusual, truncated N-glycosylation pattern. These features are linked to eliciting more robust immunological responses in animals, offering insights into CCH's enhanced immunostimulatory properties and opening new avenues for its potential applications in biomedical research and therapies.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.str.2024.02.018
dc.identifier.eissn1878-4186
dc.identifier.issn0969-2126
dc.identifier.urihttps://doi.org/10.1016/j.str.2024.02.018
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90421
dc.identifier.wosidWOS:001263750700001
dc.issue.numero6
dc.language.isoen
dc.revistaStructure
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleStructural insights into a functional unit from an immunogenic mollusk hemocyanin
dc.typeartículo
dc.volumen32
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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