In vitro regeneration of Minthostachys andina (Brett) Epling - a Bolivian native species with aromatic and medicinal properties

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dc.contributor.authorCastillo, JA
dc.contributor.authorJordan, M
dc.date.accessioned2025-01-21T01:33:40Z
dc.date.available2025-01-21T01:33:40Z
dc.date.issued1997
dc.description.abstractVarious explants of Minthostachys andina (Brett.) were evaluated for their morphogenic potential under in vitro culture conditions. Axillary buds derived from 2 year-old plants grown in MS-medium supplemented with 4.4 or 8.8 mu M BA and 0.054 mu M NAA, initiated shoot growth and new shoot formation. Under subculture in NN medium, shoots were rooted in the presence of NAA (1.6, 2.7 or 5.3 mu M) alone or in combination with IBA (9.8 mu M), and the regenerated plantlets were later acclimatised in the greenhouse. Also, polynodal segments from seedlings initiated multiple shoots and plantlets when initially cultured in presence of NN-liquid salt medium supplemented with 2.2-17.7 mu M BA or 4.5-13.6 M TDZ in combination with different auxin-like growth regulators and after a final transfer for root initiation. The same types of responses were found in hypocotyl and leaf explants, which produced adventitious shoots in the presence of TDZ. The use of antioxidants helped to prevent browning and favoured organogenesis.
dc.fuente.origenWOS
dc.identifier.issn0167-6857
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97453
dc.identifier.wosidWOS:A1997XV40800014
dc.issue.numero2
dc.language.isoen
dc.pagina.final160
dc.pagina.inicio157
dc.revistaPlant cell tissue and organ culture
dc.rightsacceso restringido
dc.subjectin vitro regeneration
dc.subjectorganogenesis
dc.subjectclonal propagation
dc.subjectessential oils
dc.subjectLabiatae
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleIn vitro regeneration of Minthostachys andina (Brett) Epling - a Bolivian native species with aromatic and medicinal properties
dc.typeartículo
dc.volumen49
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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