Comparison of features of human lung cancer cell lines and their corresponding tumors

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dc.contributor.authorWistuba, II
dc.contributor.authorBryant, D
dc.contributor.authorBehrens, C
dc.contributor.authorMilchgrub, S
dc.contributor.authorVirmani, AK
dc.contributor.authorAshfaq, R
dc.contributor.authorMinna, JD
dc.contributor.authorGazdar, AF
dc.date.accessioned2024-01-10T13:11:55Z
dc.date.available2024-01-10T13:11:55Z
dc.date.issued1999
dc.description.abstractAlthough human lung tumor-derived cell lines play an important role in the investigation of lung cancer biology and genetics, there is no comprehensive study comparing the genotypic and phenotypic properties of lung cancer cell lines with those of the individual tumors from which they were derived, We compared a variety of properties of 12 human non-small cell lung carcinoma (NSCLC) cell lines (cultured for a median period of 39 months; range, 12-69) and their corresponding archival tumor tissues. There was, in general, an excellent concordance between the lung tumor cell lines and their corresponding tumor tissues for morphology (100%), the presence of aneuploidy (100%), immunohistochemical expression of HER2/neu (100%) and p53 proteins (100%), loss of heterozygosity at 13 chromosomal regions analyzed (97%) using 37 microsatellite markers, microsatellite alterations (MAs, 75%), TP53 (67%), and K-ras (100%) gene mutations, In addition, there was 100% concordance for the parental allele lost in all 115 comparisons of allelic losses. Some discrepancies were found; more aneuploid subpopulations of cells were detected in the cell lines as well as higher incidences of TP53 mutations (4 of 10 mutations not found in the tumors) and microsatellite alterations (two cell lines with MAs not detected in the tumors). Similar loss of heterozygosity frequencies by chromosomal regions and mean fractional allelic loss index were detected between successfully cultured and 40 uncultured lung tumors (0.45 and 0.49, respectively), indicating that both groups were similar. Our findings indicate that the NSCLC cell lines in the large majority of instances retain the properties of their parental tumors for lengthy culture periods. NSCLC cell lines appear very representative of the lung cancer tumor from which they were derived and thus provide suitable model systems for biomedical studies of this important neoplasm.
dc.description.funderNATIONAL CANCER INSTITUTE
dc.description.funderNCI NIH HHS
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.eissn1557-3265
dc.identifier.issn1078-0432
dc.identifier.pubmedidMEDLINE:10353731
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78115
dc.identifier.wosidWOS:000080528300007
dc.information.autorucMedicina;Wistuba I;S/I;100278
dc.issue.numero5
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final1000
dc.pagina.inicio991
dc.publisherAMER ASSOC CANCER RESEARCH
dc.revistaCLINICAL CANCER RESEARCH
dc.rightsregistro bibliográfico
dc.subjectMICROSATELLITE INSTABILITY
dc.subjectBREAST-CANCER
dc.subjectCHROMOSOME 3P
dc.subjectEXPRESSION
dc.subjectPATHOGENESIS
dc.subjectCARCINOMA
dc.subjectMUTATIONS
dc.subjectPROTEIN
dc.subjectGENE
dc.subjectDELETIONS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleComparison of features of human lung cancer cell lines and their corresponding tumors
dc.typeartículo
dc.volumen5
sipa.codpersvinculados100278
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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