Genome-wide allelotyping analysis reveals multiple sites of allelic loss in gallbladder carcinoma

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dc.contributor.authorWistuba, II
dc.contributor.authorTang, MY
dc.contributor.authorMaitra, A
dc.contributor.authorAlvarez, H
dc.contributor.authorTroncoso, P
dc.contributor.authorPimental, F
dc.contributor.authorGazdar, AF
dc.date.accessioned2024-01-10T13:48:47Z
dc.date.available2024-01-10T13:48:47Z
dc.date.issued2001
dc.description.abstractAlthough gallbladder carcinoma (GBC) is a highly malignant neoplasm, there is very limited information about the molecular changes involved in its pathogenesis. To identify the chromosomal locations of putative tumor suppressor gene loci Involved in the pathogenesis of GBC, we conducted a genome-wide allelotyping or loss of heterozygosity (LOH) analysis of GBCs. Microdissected tissue from 24 archival GBCs and their matched control DNAs were analyzed for PCR-based LOH using 169 microsatellite markers spanning all nonacrocentric autosomal arms and the X chromosome. The chromosomal arms with the greatest frequencies of LOH (greater than or equal to 60%) were 3p, 6q, 7q, 8p, 9p, 9q, 11q, 12q, 17p, 18q, 19p, 22q, and Xq. The average fractional allele loss index in GBC cases was high (0.43) and frequent breakpoints were detected in gallbladder tumors. Of interest, 21 different regions of frequent LOH (hot spots) defined as greater than or equal to 50% for individual GBC samples were detected in this neoplasm, nearly half of them confined to one microsatellite marker. We conclude that in GBC at least 21 chromosomal regions with frequent allele losses are involved, suggesting that several putative tumor suppressor genes are inactivated in its pathogenesis. Overall, these data provide global estimates of the extent of genetic changes leading to GBC and will be useful for the identification of new tumor suppressor genes and for multiple new markers for translational research.
dc.fechaingreso.objetodigital2024-06-25
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.issn0008-5472
dc.identifier.pubmedidMEDLINE:11325854
dc.identifier.wosidWOS:000168485400042
dc.information.autorucMedicina;Wistuba I;S/I;100278
dc.issue.numero9
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final3800
dc.pagina.inicio3795
dc.publisherAMER ASSOC CANCER RESEARCH
dc.revistaCANCER RESEARCH
dc.rightsacceso restringido
dc.subjectHUMAN LUNG-CANCER
dc.subjectHUMAN HEPATOCELLULAR-CARCINOMA
dc.subjectTUMOR-SUPPRESSOR GENE
dc.subjectHIGH-RISK AREA
dc.subjectCOLORECTAL CARCINOMAS
dc.subjectTRACT CANCERS
dc.subjectHETEROZYGOSITY
dc.subjectFREQUENT
dc.subjectREGIONS
dc.subjectDELETION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGenome-wide allelotyping analysis reveals multiple sites of allelic loss in gallbladder carcinoma
dc.typeartículo
dc.volumen61
sipa.codpersvinculados100278
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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