Glutaredoxin GRXS13 plays a key role in protection against photooxidative stress in Arabidopsis

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dc.contributor.authorLaporte, Daniel
dc.contributor.authorOlate, Ema
dc.contributor.authorSalinas, Paula
dc.contributor.authorSalazar, Marcela
dc.contributor.authorJordana, Xavier
dc.contributor.authorHoluigue, Loreto
dc.date.accessioned2024-01-10T12:39:15Z
dc.date.available2024-01-10T12:39:15Z
dc.date.issued2012
dc.description.abstractGlutaredoxins (GRXs) belong to the antioxidant and signalling network involved in the cellular response to oxidative stress in bacterial and eukaryotic cells. In spite of the high number of GRX genes in plant genomes, the biological functions and physiological roles of most of them remain unknown. Here the functional characterization of the Arabidopsis GRXS13 gene (At1g03850), that codes for two CC-type GRX isoforms, is reported. The transcript variant coding for the GRXS13.2 isoform is predominantly expressed under basal conditions and is the isoform that is induced by photooxidative stress. Transgenic lines where the GRXS13 gene has been knocked down show increased basal levels of superoxide radicals and reduced plant growth. These lines also display reduced tolerance to methyl viologen (MeV) and high light (HL) treatments, both conditions of photooxidative stress characterized by increased production of superoxide ions. Consistently, lines overexpressing the GRXS13.2 variant show reduced MeV- and HL-induced damage. Alterations in GRXS13 expression also affect superoxide levels and the ascorbate/dehydroascorbate ratio after HL-induced stress. These results indicate that GRXS13 gene expression is critical for limiting basal and photooxidative stress-induced reactive oxygen species (ROS) production. Together, these results place GRXS13.2 as a member of the ROS-scavenging/antioxidant network that shows a particularly low functional redundancy in the Arabidopsis GRX family.
dc.description.funderNational Commission for Science and Technology CONICYT [FONDECYT]
dc.description.funderMillennium Science Initiative [Nucleus for Plant Functional Genomics]
dc.description.funderCONICYT
dc.fechaingreso.objetodigital2024-04-26
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1093/jxb/err301
dc.identifier.issn0022-0957
dc.identifier.pubmedidMEDLINE:21963612
dc.identifier.urihttps://doi.org/10.1093/jxb/err301
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77166
dc.identifier.wosidWOS:000298383400039
dc.information.autorucCiencias Biológicas;Holuigue L ;S/I;50349
dc.information.autorucCiencias Biológicas;Jordana X;S/I;99673
dc.information.autorucCiencias Biológicas;Laporte D ;S/I;169818
dc.information.autorucCiencias Biológicas;Olate E ;S/I;149775
dc.information.autorucCiencias Biológicas;Salazar M ;S/I;1006666
dc.information.autorucCiencias Biológicas;Salinas P ;S/I;9386
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final515
dc.pagina.inicio503
dc.publisherOXFORD UNIV PRESS
dc.revistaJOURNAL OF EXPERIMENTAL BOTANY
dc.rightsacceso abierto
dc.subjectArabidopsis
dc.subjectglutaredoxin
dc.subjectGRXS13
dc.subjecthigh light stress
dc.subjectmethyl viologen
dc.subjectoxidative stress response
dc.subjectphotooxidative stress
dc.subjectWATER-WATER CYCLE
dc.subjectASCORBATE PEROXIDASE
dc.subjectOXIDATIVE STRESS
dc.subjectPLANT GLUTAREDOXIN
dc.subjectCELL-DEATH
dc.subjectCHLOROPLASTIC GLUTAREDOXIN
dc.subjectMONOTHIOL GLUTAREDOXIN
dc.subjectSUPEROXIDE DISMUTASES
dc.subjectELECTRON-TRANSPORT
dc.subjectANTHER DEVELOPMENT
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGlutaredoxin GRXS13 plays a key role in protection against photooxidative stress in Arabidopsis
dc.typeartículo
dc.volumen63
sipa.codpersvinculados50349
sipa.codpersvinculados99673
sipa.codpersvinculados169818
sipa.codpersvinculados149775
sipa.codpersvinculados1006666
sipa.codpersvinculados9386
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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