Metabolic flux redistribution in <i>Corynebacterium glutamicum</i> in response to osmotic stress

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dc.contributor.authorVarela, C
dc.contributor.authorAgosin, E
dc.contributor.authorBaez, M
dc.contributor.authorKlapa, M
dc.contributor.authorStephanopoulos, G
dc.date.accessioned2025-01-21T01:10:04Z
dc.date.available2025-01-21T01:10:04Z
dc.date.issued2003
dc.description.abstractOsmotic stress constitutes a major bacterial stress factor in the soil and during industrial fermentation. In this paper, we quantified the metabolic response, in terms of metabolic flux redistribution, of a lysine-overproducing strain of Corynebacterium glutamicum grown under continuous culture, to gradually increasing osmolality. Oxygen and carbon dioxide evolution rates, and the changes in concentration of extracellular, as well as intracellular, metabolites were measured throughout the osmotic gradient. The metabolic fluxes were estimated from these measurements and from the mass balance constraints at each metabolite-node of the assumed metabolic reaction network. Our results show that formation rates of compatible solutes trehalose first and proline at a later stage of the gradient increased with osmotic stress to equilibrate the external osmotic pressure. Estimated flux distributions indicate that the observed increase in the glucose specific uptake rate with osmotic stress is channeled through the main energy generating pathways-glycolysis and the tricarboxylic acid cycle-while the flux through the pentose phosphate pathway remains constant throughout the gradient. This results in a significant increase in the net specific ATP production rate, which may possibly be used to support the higher energy requirements required for cellular maintenance at high osmolalities. Finally, nodal analysis confirmed that the PEP/pyruvate node is essentially rigid and that the glucose-6-phosphate, oxaloacetate and aketoglutarate nodes are flexible and therefore adaptable to changes in osmotic pressure in C. glutainicum.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00253-002-1120-7
dc.identifier.issn0175-7598
dc.identifier.urihttps://doi.org/10.1007/s00253-002-1120-7
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96648
dc.identifier.wosidWOS:000180876800007
dc.issue.numero5
dc.language.isoen
dc.pagina.final555
dc.pagina.inicio547
dc.revistaApplied microbiology and biotechnology
dc.rightsacceso restringido
dc.subject.ods12 Responsible Consumption and Production
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa12 Producción y consumo responsable
dc.subject.odspa03 Salud y bienestar
dc.titleMetabolic flux redistribution in <i>Corynebacterium glutamicum</i> in response to osmotic stress
dc.typeartículo
dc.volumen60
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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