Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities

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dc.article.number1069452
dc.catalogadorjca
dc.contributor.authorSalgado Salgado, Oscar
dc.contributor.authorGuajardo-Leiva, Sergio
dc.contributor.authorMoya-Beltrán, Ana
dc.contributor.authorBarbosa, Carla
dc.contributor.authorRidley, Christina
dc.contributor.authorTamayo-Leiva, Javier
dc.contributor.authorQuatrini, Raquel
dc.contributor.authorMojica, Francisco
dc.contributor.authorDíez, Beatriz
dc.date.accessioned2023-11-22T18:27:40Z
dc.date.available2023-11-22T18:27:40Z
dc.date.issued2022
dc.description.abstractThe Cas1 protein is essential for the functioning of CRISPR-Cas adaptive systems. However, despite the high prevalence of CRISPR-Cas systems in thermophilic microorganisms, few studies have investigated the occurrence and diversity of Cas1 across hot spring microbial communities. Phylogenomic analysis of 2,150 Cas1 sequences recovered from 48 metagenomes representing hot springs (42–80°C, pH 6–9) from three continents, revealed similar ecological diversity of Cas1 and 16S rRNA associated with geographic location. Furthermore, phylogenetic analysis of the Cas1 sequences exposed a broad taxonomic distribution in thermophilic bacteria, with new clades of Cas1 homologs branching at the root of the tree or at the root of known clades harboring reference Cas1 types. Additionally, a new family of casposases was identified from hot springs, which further completes the evolutionary landscape of the Cas1 superfamily. This ecological study contributes new Cas1 sequences from known and novel locations worldwide, mainly focusing on under-sampled hot spring microbial mat taxa. Results herein show that circumneutral hot springs are environments harboring high diversity and novelty related to adaptive immunity systems.
dc.description.funderANID FONDECYT
dc.description.funderANID Millennium Scientific Initiative
dc.description.funderCentro Ciencia and Vida
dc.description.funderFinanciamiento Basal para Centros Científicos y Tecnológicos de Excelencia de ANID
dc.description.funderFONDECYT
dc.description.funderGeneralitat Valenciana
dc.description.funderANID
dc.fechaingreso.objetodigital2023-11-22
dc.identifier.doi10.3389/fmicb.2022.1069452
dc.identifier.eissn1664302X
dc.identifier.issn1664 302X
dc.identifier.scopusidSCOPUS_ID:85144034490
dc.identifier.urihttps://doi.org/10.3389/fmicb.2022.1069452
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2022.1069452/full
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75400
dc.information.autorucFacultad de Ciencias Biológicas; Salgado Salgado Oscar Alexis; S/I ; 1050267
dc.language.isoen
dc.nota.accesoContenido completo
dc.relation.ispartofFrontiers in Microbiology
dc.revistaFrontiers in Microbiology
dc.rightsacceso abierto
dc.subjectCas1
dc.subjectCasposase
dc.subjectCRISPR-Cas
dc.subjectHot spring
dc.subjectPhylogenomic
dc.titleGlobal phylogenomic novelty of the Cas1 gene from hot spring microbial communities
dc.typeartículo
dc.volumen13
sipa.codpersvinculados1050267
sipa.trazabilidadORCID;2023-11-20
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