Molecular detection and characterization of<i> Bartonella</i> spp. in rodents from central and southern Chile, with emphasis on introduced rats<i> (Rattus</i> spp.)

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dc.contributor.authorSepulveda-Garcia, Paulina
dc.contributor.authorRubio, Andre, V
dc.contributor.authorSalgado, Rodrigo
dc.contributor.authorRiquelme, Maira
dc.contributor.authorBonacic, Cristian
dc.contributor.authorCanales, Nivia
dc.contributor.authorMueller, Ananda
dc.date.accessioned2025-01-20T17:36:17Z
dc.date.available2025-01-20T17:36:17Z
dc.date.issued2023
dc.description.abstractBartonella spp. was screened in 155 rodents from Chile, mainly the invasive rats Rattus norvegicus and Rattus rattus. A total of 155 spleen and 50 blood samples were analyzed through real-time PCR for Bartonella spp. (nuoG gene). Positive samples were subjected to amplification of fragment of loci gltA, rpoB and ITS by conventional PCR (cPCR). Overall, 43 spleen samples (27.7%) and 6 rodent blood samples (12%) were positive for nuoG- Bartonella spp. Positive samples were found in R. norvegicus, R. rattus, Abrothrix olivacea and Oligoryzomys longicaudatus. Bartonella spp. DNA was amplified by cPCR in 16 samples, resulting in 21 sequences (6 gltA, 5 ITS and 10 rpoB). Sequencing and phylogenic analyses identified genotypes from Rattus spp., potentially belonging to Bartonella coopersplainsensis, Bartonella henselae, Bartonella tribocorum, and an undescribed Bartonella sp. From native rodents, one sequence was identified, being related B. machadoae. In conclusion, this work describes diverse and potentially zoonotic Bartonella spp. genotypes in Rattus spp. Additionally, this is the first report of Bartonella in O. longicaudatus, including a potentially novel Bartonella genotype or species.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.cimid.2023.102026
dc.identifier.eissn1878-1667
dc.identifier.issn0147-9571
dc.identifier.urihttps://doi.org/10.1016/j.cimid.2023.102026
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91683
dc.identifier.wosidWOS:001075263200001
dc.language.isoen
dc.revistaComparative immunology microbiology and infectious diseases
dc.rightsacceso restringido
dc.subjectBartonellaceae
dc.subjectSouth America
dc.subjectRodentia
dc.subjectSmall mammals
dc.subjectZoonosis
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMolecular detection and characterization of<i> Bartonella</i> spp. in rodents from central and southern Chile, with emphasis on introduced rats<i> (Rattus</i> spp.)
dc.typeartículo
dc.volumen100
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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