Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines

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dc.contributor.authorIli, Carmen G.
dc.contributor.authorBrebi, Priscilla
dc.contributor.authorGarcia, Patricia
dc.contributor.authorLeal, Pamela
dc.contributor.authorLopez, Jaime
dc.contributor.authorTapia, Oscar
dc.contributor.authorLetelier, Pablo
dc.contributor.authorWeber, Helga
dc.contributor.authorCastillo, Jonathan
dc.contributor.authorRoa, Juan C.
dc.date.accessioned2025-01-23T21:33:39Z
dc.date.available2025-01-23T21:33:39Z
dc.date.issued2015
dc.description.abstractOverexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P<0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P<0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P<0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.
dc.fuente.origenWOS
dc.identifier.eissn0717-9367
dc.identifier.issn0717-9502
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101521
dc.identifier.wosidWOS:000362256800035
dc.issue.numero2
dc.language.isoen
dc.pagina.final646
dc.pagina.inicio638
dc.revistaInternational journal of morphology
dc.rightsacceso restringido
dc.subjectApoptosis
dc.subjectProliferation
dc.subjectCervical cancer
dc.subjectsiRNA
dc.subjectc-FLIPL
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEffects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
dc.typeartículo
dc.volumen33
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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