Poly-β-hydroxyalkanoates consumption during degradation of 2,4,6-trichlorophenol by <i>Sphingopyxis chilensis</i> S37

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dc.contributor.authorGodoy, FA
dc.contributor.authorBunster, M
dc.contributor.authorMatus, V
dc.contributor.authorAranda, C
dc.contributor.authorGonzález, B
dc.contributor.authorMartínez, MA
dc.date.accessioned2025-01-21T01:10:02Z
dc.date.available2025-01-21T01:10:02Z
dc.date.issued2003
dc.description.abstractAims: To analyse the possible effect of poly-beta -hydroxyalkanoate (PHA) consumption on 2,4,6-trichlorophenol (2,4,6-TCP) degradation during starvation by Sphingopyxis chilensis S37 strain, which stores PHAs and degrades 2,4,6-TCP.
dc.description.abstractMethods and Results: The strain was inoculated in saline solution supplemented with 2,4,6-TCP (25-400 muM). Chlorophenol degradation was followed both spectrophotometrically and by chlorine released; viable bacterial counts were also determined. Cells starved for 24, 48 or 72 h were incubated with 25 muM of 2,4,6-TCP and PHA in cells investigated by spectrofluorimetric and flow cytometry. Results demonstrated that starvation decreased the ability to degrade 2,4,6-TCP. After 72 h of starvation, degradation of 2,4,6-TCP decreased to less than 10% and the relative PHA content diminished to ca 50% during the first 24 h.
dc.description.abstractConclusion: Utilization of PHA may be an important factor for the degradation of toxic compounds, such as 2,4,6-TCP, in bacterial strains unable to use this toxic compound as carbon and energy source.
dc.description.abstractSignificance and Impact of the Study: This is the first study describing a relationship between intracellular PHA consumption and 2,4,6-TCP degradation. Therefore, PHAs provides an endogenous carbon and energy source under starvation and can play a significant role in the degradation of toxic compounds.
dc.fuente.origenWOS
dc.identifier.eissn1472-765X
dc.identifier.issn0266-8254
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96645
dc.identifier.wosidWOS:000182118300012
dc.issue.numero5
dc.language.isoen
dc.pagina.final320
dc.pagina.inicio315
dc.revistaLetters in applied microbiology
dc.rightsacceso restringido
dc.subjectpoly-beta-hydroxyalkanoate
dc.subjectSphingopyxis chilensis
dc.subjectstarvation
dc.subject2,4,6-trichlorophenol degradation
dc.titlePoly-β-hydroxyalkanoates consumption during degradation of 2,4,6-trichlorophenol by <i>Sphingopyxis chilensis</i> S37
dc.typeartículo
dc.volumen36
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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