Chemically induced hypoxia promotes differential outcomes over preadipocyte- or adipocyte-macrophage communication

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dc.contributor.authorAvila-George, K.
dc.contributor.authorRamos-Olivares, K.
dc.contributor.authorVasquez-Munoz, K.
dc.contributor.authorVillanueva-Morales, V.
dc.contributor.authorReyes-Farias, M.
dc.contributor.authorQuintero, P.
dc.contributor.authorGarcia, L.
dc.contributor.authorGarcia-Diaz, D. F.
dc.date.accessioned2025-01-23T21:26:14Z
dc.date.available2025-01-23T21:26:14Z
dc.date.issued2017
dc.description.abstractExpansion of white adipose tissue induce insufficient vascularization, driving hypoxia and low-grade inflammation. Resident preadipocytes are thought to be involved. We evaluated the effects of hypoxia over preadipocytes and adipocytes, to determine which cellular type impacts the most over macrophages activation. 3T3-L1 cells were either differentiated, or maintained undifferentiated. Each group was subjected to the presence or absence of chemical hypoxia (200 mu M CoCl2) for 24 h. Conditioned media were used as treatment for murine RAW264.7 macrophages for 24 h. Gene expression of HIF-1 alpha and TNF-alpha, and the release of several markers were assessed. It was observed that culture media from hypoxic preadipocytes induced greater expression of inflammatory markers and NO release than culture media from hypoxic adipocytes, by macrophages. Gene expression correlated closer with inflammatory markers release specially on macrophages treated with conditioned media from preadipocytes. Hence, the present work highlights the importance of preadipocytes on inflammatory conditions in vitro.
dc.description.funderCONICYT, Chile
dc.fuente.origenWOS
dc.identifier.doi10.1080/13813455.2017.1285318
dc.identifier.eissn1744-4160
dc.identifier.issn1381-3455
dc.identifier.urihttps://doi.org/10.1080/13813455.2017.1285318
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101349
dc.identifier.wosidWOS:000400745200004
dc.issue.numero3
dc.language.isoen
dc.pagina.final181
dc.pagina.inicio175
dc.revistaArchives of physiology and biochemistry
dc.rightsacceso restringido
dc.subjectHIF-1 alpha
dc.subjectinflammation
dc.subjectobesity
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleChemically induced hypoxia promotes differential outcomes over preadipocyte- or adipocyte-macrophage communication
dc.typeartículo
dc.volumen123
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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