Structure and transcriptional impact of divergent repetitive elements inserted within <i>Phanerochaete chrysosporium</i> strain RP-78 genes

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dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCanessa, Paulo
dc.contributor.authorVicuna, Rafael
dc.contributor.authorStewart, Philip
dc.contributor.authorVanden Wymelenberg, Amber
dc.contributor.authorCullen, Dan
dc.date.accessioned2025-01-21T01:05:46Z
dc.date.available2025-01-21T01:05:46Z
dc.date.issued2007
dc.description.abstractWe describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Searches of the P. chrysosporium genome revealed five copies of pce1, a similar to 1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harbor pce insertions and produce incomplete transcripts. Class I elements included pcret1, an intact 8.14-kb gypsy-like retrotransposon inserted within a member of the multicopper oxidase gene family. Additionally, we describe a complex insertion of nested transposons within another putative cytochrome P450 gene. The disrupted allele lies within a cluster of > 14 genes, all of which encode family 64 cytochrome P450s. Components of the insertion include a disjoint copia-like element, pcret3, the pol domain of a second retroelement, pcret2, and a duplication of an extended ORF of unknown function. As in the case of the pce elements, pcret1 and pcret2/3 insertions are confined to single alleles, transcripts of which are truncated. The corresponding wild-type alleles are apparently unaffected. In aggregate, P. chrysosporium harbors a complex array of repetitive elements, at least five of which directly influence expression of genes within families of structurally related sequences.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00438-006-0167-z
dc.identifier.issn1617-4615
dc.identifier.urihttps://doi.org/10.1007/s00438-006-0167-z
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96010
dc.identifier.wosidWOS:000242854600005
dc.issue.numero1
dc.language.isoen
dc.pagina.final55
dc.pagina.inicio43
dc.revistaMolecular genetics and genomics
dc.rightsacceso restringido
dc.subjectPhanerochaete chrysosporium
dc.subjectretrotransposons
dc.subjectmulticopper oxidase
dc.subjectcytochrome P450
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleStructure and transcriptional impact of divergent repetitive elements inserted within <i>Phanerochaete chrysosporium</i> strain RP-78 genes
dc.typeartículo
dc.volumen277
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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