Genomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Strains Recovered from Chilean Hospitals Reveals Lineages Specific to South America and Multiple Routes for Acquisition of Antibiotic Resistance Genes

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dc.catalogadorgjm
dc.contributor.authorBrito, Barbara P.
dc.contributor.authorKoong, Jonathan
dc.contributor.authorWozniak Banchero, Aniela
dc.contributor.authorOpazo-Capurro, Andres
dc.contributor.authorTo, Joyce
dc.contributor.authorGarcia, Patricia
dc.contributor.authorHamidian, Mehrad
dc.date.accessioned2024-01-30T15:23:30Z
dc.date.available2024-01-30T15:23:30Z
dc.date.issued2022
dc.description.abstractCarbapenem-resistant Acinetobacter baumannii (CRAb) is a public health threat accounting for a significant number of hospital-acquired infections. Despite the importance of this pathogen, there is scarce literature on A. baumannii molecular epidemiology and evolutionary pathways relevant to resistance emergence in South American strains. We analyzed the genomic context of 34 CRAb isolates recovered from clinical samples between 2010 and 2013 from two hospitals in Santiago, Chile, using whole-genome sequencing. Several Institut Pasteur scheme sequence types (STs) were identified among the 34 genomes studied here, including ST1, ST15, ST79, ST162, and ST109. No ST2 (the most widespread sequence type) strain was detected. Chilean isolates were phylogenetically closely related, forming lineages specific to South America (e.g., ST1, ST79, and ST15). The genomic contexts of the resistance genes were diverse: while genes were present in a plasmid in ST15 strains, all genes were chromosomal in ST79 strains. Different variants of a small Rep_3 plasmid played a central role in the acquisition of the oxa58 carbapenem and aacC2 aminoglycoside resistance genes in ST1, ST15, and ST79 strains. The aacC2 gene along with blaTEM were found in a novel transposon named Tn6925 here. Variants of Tn7 were also found to play an important role in the acquisition of the aadA1 and dfrA1 genes. This work draws a detailed picture of the genetic context of antibiotic resistance genes in a set of carbapenem-resistant A. baumannii strains recovered from two Chilean hospitals and reveals a complex evolutionary picture of antibiotic resistance gene acquisition events via multiple routes involving several mobile genetic elements.
dc.fuente.origenORCID
dc.identifier.doi10.1128/spectrum.02463-22
dc.identifier.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-85140856250&partnerID=MN8TOARS
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81066
dc.identifier.wosidWOS:000861379000001
dc.information.autorucEscuela de Medicina; Wozniak Banchero, Aniela; 0000-0001-9559-7660; 1008612
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subjectAcinetobacter baumannii
dc.subjectAntibiotic resistance
dc.subjectCarbapenem resistance
dc.subjectChile
dc.subjectPlasmids
dc.subjectSouth America
dc.subjectST1
dc.subjectST109
dc.subjectST15
dc.subjectST162
dc.subjectST79
dc.subjectWhole-genome sequence
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGenomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Strains Recovered from Chilean Hospitals Reveals Lineages Specific to South America and Multiple Routes for Acquisition of Antibiotic Resistance Genes
dc.typeartículo
sipa.codpersvinculados1008612
sipa.trazabilidadORCID;2024-01-15
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