Effects of rumen fluid pre-incubation on in vitro proteolytic activity of enzymatic extracts from rumen microorganisms

Simple item page
dc.contributor.authorVelasquez, A.
dc.contributor.authorPichard, G.
dc.date.accessioned2024-01-10T12:05:10Z
dc.date.available2024-01-10T12:05:10Z
dc.date.issued2010
dc.description.abstractEnzymatic extracts were prepared from rumen fluid (RF) either directly after collection or after incubation in enriched media and their proteolytic activities were measured and compared The objective of RF in vitro cultivation was to augment enzymatic activity by using diverse incubation media containing different feed substrates to selectively induce microorganisms with essentially unrestricted sources of energy and N The effects on protein degradation in bovine serum albumin (BSA) and soybean meal rapeseed meal dehydrated alfalfa meal and perennial ryegrass were determined Enzymatic extracts were characterized by means of protease zymograms Two adult Holstein Friesian rumen fistulated cows were used as RF donors Standard anaerobic incubation techniques in buffer-mineral media were used and protein degradation was measured as residual true protein after enzymatic incubations Results reveal an effect of RF pre-incubation on protein degradation (P<0 0001) measured in BSA and in feed substrates and also on the hydrolysis rate (P<0 001) measured with native substrates only The enzymatic extracts showed a high potential for proteolytic degradation reaching 785 mg/g crude protein (CP) degraded in BSA after 12h of incubation and 830 mg/g CP degraded in soybean meal with 48h of incubation compared to only 654 mg/g and 414 mg/g in controls The zymograms of these extracts revealed six zones of proteolytic activity with the highest concentration of peptidases in the zone of high molecular weight (117 and 130 kDa) In vitro pre-incubation of rumen fluid in culture media enriched with energy and N sources allowed collection of enzymatic extracts with higher proteolytic activity than extracts obtained directly from fresh rumen fluid (C) 2010 Elsevier B V All rights reserved
dc.description.funderFONDECYT
dc.fechaingreso.objetodigital2024-03-19
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.anifeedsci.2010.09.003
dc.identifier.issn0377-8401
dc.identifier.urihttps://doi.org/10.1016/j.anifeedsci.2010.09.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75961
dc.identifier.wosidWOS:000285672500001
dc.information.autorucAgronomía e Ing. Forestal;Pichard G ;S/I;99161
dc.information.autorucAgronomía e Ing. Forestal;Velasquez A ;S/I;151980
dc.issue.numero3-4
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final82
dc.pagina.inicio75
dc.publisherELSEVIER SCIENCE BV
dc.revistaANIMAL FEED SCIENCE AND TECHNOLOGY
dc.rightsacceso restringido
dc.subjectRumen microbial enzymes
dc.subjectEnzymes extraction
dc.subjectIn vitro protein breakdown
dc.subjectZymograms
dc.subjectPROTEIN-DEGRADATION
dc.subjectDEGRADABILITY
dc.subjectNITROGEN
dc.subjectFEEDS
dc.subjectSYSTEM
dc.subjectRATES
dc.subjectFIBER
dc.subjectGRASS
dc.subjectUREA
dc.subjectBAG
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleEffects of rumen fluid pre-incubation on in vitro proteolytic activity of enzymatic extracts from rumen microorganisms
dc.typeartículo
dc.volumen162
sipa.codpersvinculados99161
sipa.codpersvinculados151980
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2024-03-19. Effects of rumen fluid pre-incubation on in vitro proteolytic activity of enzymatic extracts from rumen microorganisms.pdf
Size:
135.14 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas