Genome-scale metabolic models of <i>Microbacterium</i> species isolated from a high altitude desert environment

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dc.contributor.authorMandakovic, Dinka
dc.contributor.authorCintolesi, Angela
dc.contributor.authorMaldonado, Jonathan
dc.contributor.authorMendoza, Sebastian N.
dc.contributor.authorAite, Meziane
dc.contributor.authorGaete, Alexis
dc.contributor.authorSaitua, Francisco
dc.contributor.authorAllende, Miguel
dc.contributor.authorCambiazo, Veronica
dc.contributor.authorSiegel, Anne
dc.contributor.authorMaass, Alejandro
dc.contributor.authorGonzalez, Mauricio
dc.contributor.authorLatorre, Mauricio
dc.date.accessioned2025-01-23T19:49:06Z
dc.date.available2025-01-23T19:49:06Z
dc.date.issued2020
dc.description.abstractThe Atacama Desert is the most arid desert on Earth, focus of important research activities related to microbial biodiversity studies. In this context, metabolic characterization of arid soil bacteria is crucial to understand their survival strategies under extreme environmental stress. We investigated whether strain-specific features of two Microbacterium species were involved in the metabolic ability to tolerate/adapt to local variations within an extreme desert environment. Using an integrative systems biology approach we have carried out construction and comparison of genome-scale metabolic models (GEMs) of two Microbacterium sp., CGR1 and CGR2, previously isolated from physicochemically contrasting soil sites in the Atacama Desert. Despite CGR1 and CGR2 belong to different phylogenetic clades, metabolic pathways and attributes are highly conserved in both strains. However, comparison of the GEMs showed significant differences in the connectivity of specific metabolites related to pH tolerance and CO2 production. The latter is most likely required to handle acidic stress through decarboxylation reactions. We observed greater GEM connectivity within Microbacterium sp. CGR1 compared to CGR2, which is correlated with the capacity of CGR1 to tolerate a wider pH tolerance range. Both metabolic models predict the synthesis of pigment metabolites (beta -carotene), observation validated by HPLC experiments. Our study provides a valuable resource to further investigate global metabolic adaptations of bacterial species to grow in soils with different abiotic factors within an extreme environment.
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41598-020-62130-8
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/s41598-020-62130-8
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100481
dc.identifier.wosidWOS:000560406800008
dc.issue.numero1
dc.language.isoen
dc.revistaScientific reports
dc.rightsacceso restringido
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleGenome-scale metabolic models of <i>Microbacterium</i> species isolated from a high altitude desert environment
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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