Transcriptome analysis of the response to thyroid hormone in <i>Xenopus</i> neural stem and progenitor cells

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dc.contributor.authorCordero-Veliz, Camila
dc.contributor.authorLarrain, Juan
dc.contributor.authorFaunes, Fernando
dc.date.accessioned2025-01-20T20:20:52Z
dc.date.available2025-01-20T20:20:52Z
dc.date.issued2023
dc.description.abstractBackground The thyroid hormones-thyroxine (T4) and 3,5,3 ' triiodothyronine (T3)-regulate the development of the central nervous system (CNS) in vertebrates by acting in different cell types. Although several T3 target genes have been identified in the brain, the changes in the transcriptome in response to T3 specifically in neural stem and progenitor cells (NSPCs) during the early steps of NSPCs activation and neurogenesis have not been studied in vivo. Here, we characterized the transcriptome of FACS-sorted NSPCs in response to T3 during Xenopus laevis metamorphosis. Results We identified 1252 upregulated and 726 downregulated genes after 16 hours of T3 exposure. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that T3-upregulated genes were significantly enriched in rRNA processing and maturation, protein folding, ribosome biogenesis, translation, mitochondrial function, and proteasome. These results suggest that NSPCs activation induced by T3 is characterized by an early proteome remodeling through the synthesis of the translation machinery and the degradation of proteins by the proteasome. Conclusion This work provides new insights into the dynamics of activation of NPSCs in vivo in response to T3 during a critical period of neurogenesis in the metamorphosis.
dc.fuente.origenWOS
dc.identifier.doi10.1002/dvdy.535
dc.identifier.eissn1097-0177
dc.identifier.issn1058-8388
dc.identifier.urihttps://doi.org/10.1002/dvdy.535
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92600
dc.identifier.wosidWOS:000855000000001
dc.issue.numero2
dc.language.isoen
dc.pagina.final304
dc.pagina.inicio294
dc.revistaDevelopmental dynamics
dc.rightsacceso restringido
dc.subjectmitochondria
dc.subjectneural stem and progenitor cells
dc.subjectproteasome
dc.subjectribosome biogenesis
dc.subjectthyroid hormone
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTranscriptome analysis of the response to thyroid hormone in <i>Xenopus</i> neural stem and progenitor cells
dc.typeartículo
dc.volumen252
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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