Development and pH sensitivity of the respiratory rhythm of fetal mice in vitro

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dc.contributor.authorEugenin, J.
dc.contributor.authorVon Bernhardi, R.
dc.contributor.authorMuller, K. J.
dc.contributor.authorLlona, I.
dc.date.accessioned2024-01-10T13:12:16Z
dc.date.available2024-01-10T13:12:16Z
dc.date.issued2006
dc.description.abstractIn newborn and adult mammals, chemosensory drive exerted by CO2 and H+ provides an essential tonic input: without it the rhythm of respiration is abolished. It is not known, however, whether this chemosensory drive and the respiratory rhythm appear simultaneously during development. In isolated brainstem-spinal cord preparations from fetal mice, we determined at what stage of fetal life the respiratory rhythm appeared in third to fifth cervical ventral roots (phrenic motoneurons) and whether this fetal rhythm was sensitive to chemosensory inputs. A respiratory-like rhythm consisting of short duration bursts of discharges recurring at 2-16 min(-1) was detected in two of nine embryonic day 13 fetuses; it was abolished by transection of the spinal cord between the first to second cervical segments and was phase-related to rhythmic activity from medullary units of the ventral respiratory group. At embryonic day 13, it coexisted with a slow rhythm (0.1-2.0 min(-1)) of long duration bursts of action potentials which was generated by the spinal cord. At later fetal stages, the respiratory-like rhythm became more robust and of higher frequency, while the spinal cord rhythm became less obvious. At all fetal stages, acidification of the superfusion medium from pH 7.5-7.2 or 7.4-7.3 or 7.4 to 7.2 increased the frequency of both the respiratory-like and the spinal cord rhythms. In addition, acidification reduced the amplitude of the integrated burst activity of the spinal cord rhythm of embryonic day 13-embryonic day 16 fetuses and the respiratory-like rhythm of embryonic day 17 and older fetuses. Our results indicate that the rhythms transmitted by phrenic motoneurons during fetal development are chemosensitive from early fetal stages. Through its effects on induction and patterning of the rhythm, chemosensory drive may play a role in activity-dependent formation of respiratory neural networks. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
dc.fechaingreso.objetodigital03-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.neuroscience.2006.03.046
dc.identifier.eissn1873-7544
dc.identifier.issn0306-4522
dc.identifier.pubmedidMEDLINE:16675136
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2006.03.046
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78163
dc.identifier.wosidWOS:000239033300022
dc.information.autorucMedicina;Von Bernhardi R;S/I;62523
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final231
dc.pagina.inicio223
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.revistaNEUROSCIENCE
dc.rightsacceso restringido
dc.subjectcircuitry and pattern generation
dc.subjectcentral chemoreception
dc.subjectneural control
dc.subjectrespiratory rhythm
dc.subjectSTEM-SPINAL-CORD
dc.subjectBRAIN-STEM
dc.subjectFICTIVE RESPIRATION
dc.subjectNEWBORN MOUSE
dc.subjectCO2 DIALYSIS
dc.subjectMEDULLARY
dc.subjectRAT
dc.subjectNEURONS
dc.subjectPATTERNS
dc.subjectCHEMOSENSITIVITY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDevelopment and pH sensitivity of the respiratory rhythm of fetal mice in vitro
dc.typeartículo
dc.volumen141
sipa.codpersvinculados62523
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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