Early requirement of Hyaluronan for tail regeneration in Xenopus tadpoles

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dc.contributor.authorContreras, Esteban G.
dc.contributor.authorGaete, Marcia
dc.contributor.authorSanchez, Natalia
dc.contributor.authorCarrasco, Hector
dc.contributor.authorLarrain, Juan
dc.date.accessioned2024-01-10T12:38:28Z
dc.date.available2024-01-10T12:38:28Z
dc.date.issued2009
dc.description.abstractTail regeneration in Xenopus tadpoles is a favorable model system to understand the molecular and cellular basis of tissue regeneration. Although turnover of the extracellular matrix (ECM) is a key event during tissue injury and repair, no functional studies to evaluate its role in appendage regeneration have been performed. Studying the role of Hyaluronan (HA), an ECM component, is particularly attractive because it can activate intracellular signaling cascades after tissue injury. Here we studied the function of HA and components of the HA pathway in Xenopus tadpole tail regeneration. We found that transcripts for components of this pathway, including Hyaluronan synthase2 (HAS2), Hyaluronidase2 and its receptors CD44 and RHAMM, were transiently upregulated in the regenerative bud after tail amputation. Concomitantly, an increase in HA levels was observed. Functional experiments using 4-methylumbelliferone, a specific HAS inhibitor that blocked the increase in HA levels after tail amputation, and transgenesis demonstrated that the HA pathway is required during the early phases of tail regeneration. Proper levels of HA are required to sustain proliferation of mesenchymal cells in the regenerative bud. Pharmacological and genetic inhibition of GSK3 beta was sufficient to rescue proliferation and tail regeneration when HA synthesis was blocked, suggesting that GSK3 beta is downstream of the HA pathway. We have demonstrated that HA is an early component of the regenerative pathway and is required for cell proliferation during the early phases of Xenopus tail regeneration. In addition, a crosstalk between HA and GSK3 beta signaling during tail regeneration was demonstrated.
dc.description.funderCONICYT
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1242/dev.035501
dc.identifier.eissn1477-9129
dc.identifier.issn0950-1991
dc.identifier.pubmedidMEDLINE:19666825
dc.identifier.urihttps://doi.org/10.1242/dev.035501
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77048
dc.identifier.wosidWOS:000268775400013
dc.information.autorucCiencias Biológicas;Carrasco H;S/I;7258
dc.information.autorucCiencias Biológicas;Contreras EG;S/I;132639
dc.information.autorucMedicina;Gaete M;S/I;1007357
dc.information.autorucCiencias Biológicas;Larraín J;S/I;90468
dc.information.autorucCiencias Biológicas;Sánchez N;S/I;149693
dc.issue.numero17
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final2996
dc.pagina.inicio2987
dc.publisherCOMPANY OF BIOLOGISTS LTD
dc.revistaDEVELOPMENT
dc.rightsacceso restringido
dc.subjectXenopus
dc.subjectAppendage regeneration
dc.subjectHyaluronan
dc.subjectGSK3 beta
dc.subjectGLYCOGEN-SYNTHASE KINASE-3
dc.subjectI-SCEI MEGANUCLEASE
dc.subjectEXTRACELLULAR-MATRIX
dc.subjectSIGNALING PATHWAYS
dc.subjectLIMB REGENERATION
dc.subjectCELL-MIGRATION
dc.subjectTISSUE-INJURY
dc.subjectSTEM-CELLS
dc.subjectBETA
dc.subjectWNT
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEarly requirement of Hyaluronan for tail regeneration in Xenopus tadpoles
dc.typeartículo
dc.volumen136
sipa.codpersvinculados7258
sipa.codpersvinculados132639
sipa.codpersvinculados1007357
sipa.codpersvinculados90468
sipa.codpersvinculados149693
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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