Structural characterization of the saxitoxin-targeting APTSTX1 aptamer using optical tweezers and molecular dynamics simulations

Simple item page
dc.contributor.authorCasanova-Morales, Nathalie
dc.contributor.authorFigueroa, Nataniel L.
dc.contributor.authorAlfaro, Karol
dc.contributor.authorMontenegro, Felipe
dc.contributor.authorBarrera, Nelson P.
dc.contributor.authorMaze, J. R.
dc.contributor.authorWilson, Christian A. M.
dc.contributor.authorConejeros, Pablo
dc.date.accessioned2025-01-23T19:57:33Z
dc.date.available2025-01-23T19:57:33Z
dc.date.issued2019
dc.description.abstractOptical tweezers have enabled the exploration of picoNewton forces and dynamics in single-molecule systems such as DNA and molecular motors. In this work, we used optical tweezers to study the folding/unfolding dynamics of the APTSTX1-aptamer, a single-stranded DNA molecule with high affinity for saxitoxin (STX), a lethal neurotoxin. By measuring the transition force during (un)folding processes, we were able to characterize and distinguish the conformational changes of this aptamer in the presence of magnesium ions and toxin. This work was supported by molecular dynamics (MD) simulations to propose an unfolding mechanism of the aptamer-Mg+2 complex. Our results are a step towards the development of new aptamer-based STX sensors that are potentially cheaper and more sensitive than current alternatives.
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0222468
dc.identifier.issn1932-6203
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0222468
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100741
dc.identifier.wosidWOS:000532694400008
dc.issue.numero11
dc.language.isoen
dc.revistaPlos one
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleStructural characterization of the saxitoxin-targeting APTSTX1 aptamer using optical tweezers and molecular dynamics simulations
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas