High D-glucose reduces promoter activity of human equilibrative nucleoside transporter 1 in human umbilical vein endothelium

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dc.contributor.authorFarías Jofré, Marcelo Enrique
dc.contributor.authorSan Martin, R
dc.contributor.authorSobrevía Luarte, Luis Alberto
dc.date.accessioned2023-06-30T20:35:11Z
dc.date.available2023-06-30T20:35:11Z
dc.date.issued2006
dc.description.abstractReduction of adenosine uptake by human equilibrative membrane transporters 1 (hENT1) in human umbilical vein endothelial cells (HUVEC) from gestational diabetes, or in HUVEC from normal pregnancies exposed to high extracellular D-glucose, is associated with reduced hENT1 mRNA expression. We studied the effect of high D-glucose on the transcriptional activity of the promoter region of SLC29A1 gene (for hENT1) in HUVEC. Methods: Cells were isolated and cultured in medium 199 (Ethics committee approval and informed patient consent were obtained). Fragments of SLC29A1 promoter (-3100, -2056, -1016 and -697 bp from ATG) were subcloned in pGL3 vector, upstream firefly luciferase reporter gene. Cells were co-transfected with hENT1-promoter constructs and pRL-TK vector by electroporation (320 V, 20 ms) and exposed to 5 or 25 mM D-glucose (24 hrs). Results: firefly/renilla luciferase activity was similar in all constructs transfected in 5 mM D-glucose. However, 25 mM D-glucose was associated with reduced transcriptional activity of sequences -697 to -1016 bp and -2056 to -3100 bp. Conclusions: These results suggest that the reduced hENT1 mRNA level detected in HUVEC exposed to high D-glucose could result from altered transcriptional activity of SLC29A1 promoter, most likely related to activation of repressor sequences of this gene.
dc.fechaingreso.objetodigital2023-06-13
dc.format.extent1 página
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.placenta.2005.10.001
dc.identifier.issn0143-4004
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2005.10.001
dc.identifier.urihttps://publons.com/wos-op/publon/18766758/
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74012
dc.information.autorucEscuela de Medicina ; Farías Jofré, Marcelo Enrique ; 0000-0003-0473-2295 ; 12286
dc.information.autorucEscuela de Medicina ; Sobrevía Luarte, Luis Alberto ; 0000-0001-5802-2243 ; 1002656
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.finalA41
dc.pagina.inicioA41
dc.relation.ispartof2° Latinamerican Symposium on Fetal-Maternal Interaction and Placenta and XIX Annual Meeting of The Chilean Society of Physiological Sciences (2005 ; Santiago ; Chile)
dc.revistaPlacenta
dc.rightsacceso restringido
dc.titleHigh D-glucose reduces promoter activity of human equilibrative nucleoside transporter 1 in human umbilical vein endothelium
dc.typecomunicación de congreso
dc.volumen27
sipa.codpersvinculados12286
sipa.codpersvinculados1002656
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