Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Genes in Enterobacterales, Pseudomonas and Acinetobacter Isolates from the Uterus of Healthy Mares

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dc.contributor.authorThomson, Pamela
dc.contributor.authorGarcia, Patricia
dc.contributor.authordel Rio, Camila
dc.contributor.authorCastro, Rodrigo
dc.contributor.authorNunez, Andrea
dc.contributor.authorMiranda, Carolina
dc.date.accessioned2025-01-20T17:37:13Z
dc.date.available2025-01-20T17:37:13Z
dc.date.issued2023
dc.description.abstractAntibiotic-resistant bacteria are a growing concern for human and animal health. The objective of this study was to determine the antimicrobial resistance and extended-spectrum beta-lactamase genes in Enterobacterales, Pseudomonas spp. and Acinetobacter spp. isolates from the uterus of healthy mares. For this purpose, 21 mares were swabbed for samples, which were later seeded on blood agar and MacConkey agar. The isolates were identified using MALDI-TOF and the antimicrobial susceptibility test was performed using the Kirby-Bauer technique. To characterize the resistance genes, a polymerase chain reaction (PCR) scheme was performed. Of the isolates identified as Gram-negative, 68.8% were Enterobacterales, represented by E. coli, Enterobacter cloacae, Citrobacter spp., and Klebsiella pneumoniae; 28.1% belonged to the genus Acinetobacter spp.; and 3.1% to Pseudomonas aeruginosa. A 9.3% of the isolates were multidrug-resistant (MDR), presenting resistance to antibiotics from three different classes, while 18.8% presented resistance to two or more classes of different antibiotics. The diversity of three genes that code for ESBL (bla(TEM), bla(CTX-M) and bla(SHV)) was detected in 12.5% of the strains. The most frequent was bla(SHV), while bla(TEM) and bla(CTX-M) were present in Citrobacter spp. and Klebsiella pneumoniae. These results are an alarm call for veterinarians and their environment and suggest taking measures to prevent the spread of these microorganisms.
dc.description.funderThe authors thank ANID, PAI project # 77190079, for the financial support given to this project.
dc.fuente.origenWOS
dc.identifier.doi10.3390/pathogens12091145
dc.identifier.eissn2076-0817
dc.identifier.urihttps://doi.org/10.3390/pathogens12091145
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91692
dc.identifier.wosidWOS:001074045900001
dc.issue.numero9
dc.language.isoen
dc.revistaPathogens
dc.rightsacceso restringido
dc.subjectuterus
dc.subjectmares
dc.subjectantibiotic resistance
dc.subjectESBL
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAntimicrobial Resistance and Extended-Spectrum Beta-Lactamase Genes in Enterobacterales, Pseudomonas and Acinetobacter Isolates from the Uterus of Healthy Mares
dc.typeartículo
dc.volumen12
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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