Two identical triplet sisters carrying a germline BRCAI gene mutation acquire very similar breast cancer somatic mutations at multiple other sites throughout the genome

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dc.contributor.authorWistuba, II
dc.contributor.authorTomlinson, GE
dc.contributor.authorBehrens, C
dc.contributor.authorVirmani, A
dc.contributor.authorGeradts, J
dc.contributor.authorBlum, JL
dc.contributor.authorMinna, JD
dc.contributor.authorGazdar, AF
dc.date.accessioned2024-01-10T13:44:02Z
dc.date.available2024-01-10T13:44:02Z
dc.date.issued2000
dc.description.abstractMonozygotic twins, each of whom has breast cancer, offer a natural study population for gene-environmental interactions as causation of cancer, because they are genetically identical. If heritable factors play a large role in the origin of a neoplasm, disease concordance should be significant in monozygotic twins. Two monozygotic triplet sisters carrying a germline BRCA1 gene mutation (5382insC) who both developed breast cancer at early ages were studied for loss of heterozygosity (LOH) in their microdissected, paraffin-embedded tumors along with control blood and stromal breast tissue at 19 chromosomal arms using 161 microsatellite markers. Microdissected areas of normal lobular and ductal epithelium and ductal in situ carcinoma were also studied for LOH using a subset of microsatellite markers. The mother's DNA (extracted from peripheral blood lymphocytes) was analyzed to determine the parental allele under LOH in each case. Both tumors demonstrated similar histologic features suggestive of a secretory variant of ductal carcinoma. The tumors from both sisters had similar overall LOH frequency expressed by the fractional allelic loss (FAL) indices (0.56 vs, 0.60) and demonstrated concordance for loss or retention at 82 of 97 informative markers (85% correlation). In addition, detailed mapping analysis of several chromosomal arms revealed that identical breakpoints were detected in both tumors at several chromosome regions. Finally, in both sisters' tumors, when a chromosome exhibited allelic loss, all of the markers exhibited LOH of the same parental allele even when there were intervening regions of retention of heterozygosity, In contrast, 17 archival sporadic breast carcinomas demonstrated a wide range of FAL indexes and highly individual patterns of LOH. Our findings support the hypothesis that inherited factors play a role in the development of the multiple somatic deletions occurring in breast carcinomas. Whether one of these factors is the mutant BRCA1 allele or some other gene(s) remains to be determined. (C) 2000 Wiley-Liss, Inc.
dc.description.funderNCI NIH HHS
dc.description.funderNATIONAL CANCER INSTITUTE
dc.fechaingreso.objetodigital03-04-2024
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/1098-2264(200008)28:4<359
dc.identifier.eissn1098-2264
dc.identifier.issn1045-2257
dc.identifier.pubmedidMEDLINE:10862044
dc.identifier.urihttps://doi.org/10.1002/1098-2264(200008)28:4<359
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78824
dc.identifier.wosidWOS:000088021700001
dc.information.autorucMedicina;Wistuba I;S/I;100278
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final369
dc.pagina.inicio359
dc.publisherWILEY
dc.revistaGENES CHROMOSOMES & CANCER
dc.rightsacceso restringido
dc.subjectCHROMOSOME 3P
dc.subjectHETEROZYGOSITY
dc.subjectCARCINOMA
dc.subjectTUMOR
dc.subjectEXPRESSION
dc.subjectCELLS
dc.subjectIDENTIFICATION
dc.subjectCARCINOGENESIS
dc.subjectPATHOGENESIS
dc.subjectMECHANISMS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTwo identical triplet sisters carrying a germline BRCAI gene mutation acquire very similar breast cancer somatic mutations at multiple other sites throughout the genome
dc.typeartículo
dc.volumen28
sipa.codpersvinculados100278
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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