Role of the Wnt receptor Frizzled-1 in presynaptic differentiation and function

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dc.contributor.authorVarela Nallar, Lorena Patricia
dc.contributor.authorGrabowski, Catalina P.
dc.contributor.authorAlfaro, Iván E.
dc.contributor.authorÁlvarez Rojas, Alejandra
dc.contributor.authorInestrosa Cantín, Nibaldo
dc.date.accessioned2019-10-17T18:22:01Z
dc.date.available2019-10-17T18:22:01Z
dc.date.issued2009
dc.date.updated2019-10-14T18:56:52Z
dc.description.abstractAbstract Background The Wnt signaling pathway regulates several fundamental developmental processes and recently has been shown to be involved in different aspects of synaptic differentiation and plasticity. Some Wnt signaling components are localized at central synapses, and it is thus possible that this pathway could be activated at the synapse. Results We examined the distribution of the Wnt receptor Frizzled-1 in cultured hippocampal neurons and determined that this receptor is located at synaptic contacts co-localizing with presynaptic proteins. Frizzled-1 was found in functional synapses detected with FM1-43 staining and in synaptic terminals from adult rat brain. Interestingly, overexpression of Frizzled-1 increased the number of clusters of Bassoon, a component of the active zone, while treatment with the extracellular cysteine-rich domain (CRD) of Frizzled-1 decreased Bassoon clustering, suggesting a role for this receptor in presynaptic differentiation. Consistent with this, treatment with the Frizzled-1 ligand Wnt-3a induced presynaptic protein clustering and increased functional presynaptic recycling sites, and these effects were prevented by co-treatment with the CRD of Frizzled-1. Moreover, in synaptically mature neurons Wnt-3a was able to modulate the kinetics of neurotransmitter release. Conclusion Our results indicate that the activation of the Wnt pathway through Frizzled-1 occurs at the presynaptic level, and suggest that the synaptic effects of the Wnt signaling pathway could be modulated by local activation through synaptic Frizzled receptors.
dc.fuente.origenBiomed Central
dc.identifier.citationNeural Development. 2009 Nov 02;4(1):41
dc.identifier.doi10.1186/1749-8104-4-41
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26830
dc.issue.numeroNo. 41
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final15
dc.pagina.inicio1
dc.revistaNeural Developmentes_ES
dc.rightsacceso abierto
dc.rights.holderVarela-Nallar et al; licensee BioMed Central Ltd.
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherNeurología - Investigacioneses_ES
dc.subject.otherSinapsises_ES
dc.subject.otherMedicinaes_ES
dc.titleRole of the Wnt receptor Frizzled-1 in presynaptic differentiation and functiones_ES
dc.typeartículo
dc.volumenVol.4
sipa.codpersvinculados127946
sipa.codpersvinculados83681
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