Female infertility due to anovulation and defective steroidogenesis in NPC2 deficient mice

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dc.contributor.authorBusso, D.
dc.contributor.authorOnate Alvarado, M. J.
dc.contributor.authorBalboa, E.
dc.contributor.authorZanlungo, S.
dc.contributor.authorMoreno, R. D.
dc.date.accessioned2024-01-10T13:13:05Z
dc.date.available2024-01-10T13:13:05Z
dc.date.issued2010
dc.description.abstractNiemann Pick C2 (NPC2) and NPC1 proteins function cooperatively to catalyze cholesterol efflux from lysosomes. NPC1 is expressed in ovarian cells and female NPC1 mice are infertile. This work addressed for the first time the localization and function of murine NPC2 protein in the ovary. Ovarian NPC2 was localized to theca and luteal cells, which use cholesterol as a substrate to produce estradiol and progesterone, respectively. NPC2 deficient (NPC2-/-) females had abnormal estrous cycles and were infertile, with normal folliculogenesis until the antral stage, but a complete absence of corpora lutea and many zonae pellucidae remnants. indicative of anovulation. Serum estradiol was reduced and ovarian cholesterol was accumulated in NPC2-/- mice, suggesting a defect in cholesterol export from intracellular stores. After superovulation, NPC2-/- mice ovulated less eggs than their wild type littermates, showed ovaries with less corpora lutea and numerous unruptured follicles, and lower serum progesterone concentration. Together, these results suggest that NPC2 participates in the traffic of ovarian cholesterol required to provide the substrate for steroid synthesis and support follicle maturation, ovulation and luteinization. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
dc.description.funderFondo de Desarrollo Cientifico y Tecnologico (FONDECYT)
dc.fechaingreso.objetodigital03-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.mce.2009.10.011
dc.identifier.issn0303-7207
dc.identifier.pubmedidMEDLINE:19883728
dc.identifier.urihttps://doi.org/10.1016/j.mce.2009.10.011
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78265
dc.identifier.wosidWOS:000274608900039
dc.information.autorucMedicina;Balboa E;S/I;142402
dc.information.autorucCiencias Biológicas;Busso D;S/I;161700
dc.information.autorucCiencias Biológicas;Moreno RD;S/I;76818
dc.information.autorucCiencias Biológicas;Oñate-Alvarado MJ;S/I;149674
dc.information.autorucMedicina;Zanlungo S;S/I;72650
dc.issue.numero1-2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final307
dc.pagina.inicio299
dc.publisherELSEVIER IRELAND LTD
dc.revistaMOLECULAR AND CELLULAR ENDOCRINOLOGY
dc.rightsacceso restringido
dc.subjectFemale fertility
dc.subjectOvary
dc.subjectOvulation
dc.subjectSteroids
dc.subjectNPC2
dc.subjectCholesterol
dc.subjectDISEASE TYPE-C
dc.subjectINTRACELLULAR CHOLESTEROL TRANSPORT
dc.subjectOVARIAN-FUNCTION
dc.subjectEXPRESSION
dc.subjectGENE
dc.subjectPROGESTERONE
dc.subjectTRAFFICKING
dc.subjectMETABOLISM
dc.subjectPREGNANCY
dc.subjectPROTEINS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFemale infertility due to anovulation and defective steroidogenesis in NPC2 deficient mice
dc.typeartículo
dc.volumen315
sipa.codpersvinculados142402
sipa.codpersvinculados161700
sipa.codpersvinculados76818
sipa.codpersvinculados149674
sipa.codpersvinculados72650
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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