The rat liver Na+/bile acid cotransporter - Importance of the cytoplasmic tail to function and plasma membrane targeting

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dc.contributor.authorSun, AQ
dc.contributor.authorArrese, MA
dc.contributor.authorZeng, L
dc.contributor.authorSwaby, I
dc.contributor.authorZhou, MM
dc.contributor.authorSuchy, FJ
dc.date.accessioned2024-01-10T12:44:06Z
dc.date.available2024-01-10T12:44:06Z
dc.date.issued2001
dc.description.abstractTo understand the potential functions of the cytoplasmic tail of Na+/taurocholate cotransporter (Ntcp) and to determine the basolateral sorting mechanisms for this transporter, green fluorescent protein-fused wild type and mutant rat Ntcps were constructed and the transport properties and cellular localization were assessed in transfected COS 7 and Madin-Darby canine kidney (MDCK) cells. Truncation of the 56-amino acid cytoplasmic tail demonstrates that the cytoplasmic tail of rat Ntcp is involved membrane delivery of this protein in nonpolarized and polarized cells and removal of the tail does not affect the bile acid transport function of Ntcp, Using site-directed mutagenesis, two tyrosine residues, Tyr-321 and Tyr-307, in the cytoplasmic tail of Ntcp have been identified as important for the basolateral sorting of rat Ntcp in transfected MDCK cells. Tyr-321 appears to be the major basolateral-sorting determinant, and Tyr-307 acts as a supporting determinant to ensure delivery of the transporter to the basolateral surface, especially at high levels of protein expression, When the two Tyr-based basolateral sorting motifs have been removed, the N-linked carbohydrate groups direct the tyrosine to alanine mutants to the apical surface of transfected MDCK cells. The major basolateral sorting determinant Tyr-321 is within a novel beta -turn unfavorable tetrapeptide Y(321)KAA, which has not been found in any naturally occurring basolateral sorting motifs, Two-dimensional NMR spectroscopy of a 24-mer peptide corresponding to the sequence from Tyr-307 to Thr-330 on the cytoplasmic tail of Ntcp confirms that both the Tyr-321 and Tyr-307 regions do not adopt any turn structure. Since the major motif YKAA contains a beta -turn unfavorable structure, the Ntcp basolateral sorting may not be related to the clathrin-adaptor complex pathway, as is the case for many basolateral proteins.
dc.description.funderNCRR NIH HHS
dc.description.funderNICHD NIH HHS
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT
dc.description.funderNATIONAL CENTER FOR RESEARCH RESOURCES
dc.fechaingreso.objetodigital2024-04-25
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1074/jbc.M008797200
dc.identifier.issn0021-9258
dc.identifier.pubmedidMEDLINE:11112779
dc.identifier.urihttps://doi.org/10.1074/jbc.M008797200
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77647
dc.identifier.wosidWOS:000167261000096
dc.information.autorucMedicina;Arrese M;S/I;76095
dc.issue.numero9
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final6833
dc.pagina.inicio6825
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.revistaJOURNAL OF BIOLOGICAL CHEMISTRY
dc.rightsacceso abierto
dc.subjectBASOLATERAL SORTING SIGNAL
dc.subjectDARBY CANINE KIDNEY
dc.subjectINTERNALIZATION SIGNAL
dc.subjectTRANSFERRIN RECEPTOR
dc.subjectEPITHELIAL-CELLS
dc.subjectTYROSINE
dc.subjectLOCALIZATION
dc.subjectDOMAIN
dc.subjectDISTINCT
dc.subjectENDOCYTOSIS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe rat liver Na+/bile acid cotransporter - Importance of the cytoplasmic tail to function and plasma membrane targeting
dc.typeartículo
dc.volumen276
sipa.codpersvinculados76095
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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