Serial analysis of gene expression identifies connective tissue growth factor expression as a prognostic biomarker in gallbladder cancer

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dc.contributor.authorAlvarez, Hector
dc.contributor.authorCorvalan, Alejandro
dc.contributor.authorRoa, Juan C.
dc.contributor.authorArgani, Pedram
dc.contributor.authorMurillo, Francisco
dc.contributor.authorEdwards, Jennifer
dc.contributor.authorBeaty, Robert
dc.contributor.authorFeldmann, Georg
dc.contributor.authorHong, Seung Mo
dc.contributor.authorMullendore, Michael
dc.contributor.authorRoa, Ivan
dc.contributor.authorIbanez, Luis
dc.contributor.authorPimente, Fernando
dc.contributor.authorDiaz, Alfonso
dc.contributor.authorRiggins, Gregory J.
dc.contributor.authorMaitra, Anirban
dc.date.accessioned2024-01-10T12:42:33Z
dc.date.available2024-01-10T12:42:33Z
dc.date.issued2008
dc.description.abstractBackground: Gallbladder cancer (GBC) is an uncommon neoplasm in the United States, but one with high mortality rates. This malignancy remains largely understudied at the molecular level such that few targeted therapies or predictive biomarkers exist.
dc.description.abstractExperimental Design: We built the first series of serial analysis of gene expression (SAGE) libraries from GBC and nonneoplastic gallbladder mucosa, composed of 21-bp long-SAGE tags. SAGE libraries were generated from three stage-matched GBC patients (representing Hispanic/ Latino, Native American, and Caucasian ethnicities, respectively) and one histologically alithiasic gallbladder. Real-time quantitative PCR was done on microdissected epithelium from five matched GBC and corresponding nonneoplastic gallbladder mucosa. Immunohistochemical analysis was done on a panel of 182 archival GBC in high-throughput tissue microarray format.
dc.description.abstractResults: SAGE tags corresponding to connective tissue growth factor (CTGF) transcripts were identified as differentially overexpressed in all pairwise comparisons of GBC (P < 0.001). Real-time quantitative PCR confirmed significant overexpression of CTGF transcripts in microdissected primary GBC (P < 0.05), but not in metastatic GBC, compared with nonneoplastic gallbladder epithelium. By immunohistochemistry, 66 of 182 (36%) GBC had high CTGF antigen labeling, which was significantly associated with better survival on univariate analysis (P = 0.0069, log-rank test).
dc.description.abstractConclusions: An unbiased analysis of the GBC transcriptome by SAGE has identified CTGF expression as a predictive biomarker of favorable prognosis in this malignancy. The SAGE libraries from GBC and nonneoplastic gallbladder mucosa are publicly available at the Cancer Genome Anatomy Project web site and should facilitate much needed research into this lethal neoplasm.
dc.description.funderNATIONAL CANCER INSTITUTE
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1158/1078-0432.CCR-07-1991
dc.identifier.eissn1557-3265
dc.identifier.issn1078-0432
dc.identifier.pubmedidMEDLINE:18451226
dc.identifier.urihttps://doi.org/10.1158/1078-0432.CCR-07-1991
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77520
dc.identifier.wosidWOS:000255616300015
dc.information.autorucMedicina;Corvalán A;S/I;63885
dc.information.autorucMedicina;Díaz A;S/I;98511
dc.information.autorucMedicina;Ibáñez L;S/I;99661
dc.information.autorucMedicina;Pimentel F;S/I;58222
dc.information.autorucBachillerato;Álvarez H;S/I;10806
dc.issue.numero9
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final2638
dc.pagina.inicio2631
dc.publisherAMER ASSOC CANCER RESEARCH
dc.revistaCLINICAL CANCER RESEARCH
dc.rightsacceso restringido
dc.subjectPANCREATIC ADENOCARCINOMA
dc.subjectTUMOR PROGRESSION
dc.subjectMETASTASIS
dc.subjectMESOTHELIN
dc.subjectMARKER
dc.subjectACTIVATION
dc.subjectCARCINOMA
dc.subjectHYPOXIA
dc.subjectANATOMY
dc.subjectGENOME
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSerial analysis of gene expression identifies connective tissue growth factor expression as a prognostic biomarker in gallbladder cancer
dc.typeartículo
dc.volumen14
sipa.codpersvinculados63885
sipa.codpersvinculados98511
sipa.codpersvinculados99661
sipa.codpersvinculados58222
sipa.codpersvinculados10806
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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