Spatiotemporal control of microtubule acetylation by mechanical cues regulates lysosome dynamics at the immune synapse of B cells to promote antigen presentation

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dc.catalogadorvzp
dc.contributor.authorDel Valle Batalla, Felipe Andres
dc.contributor.authorRiobo Cavada, Isidora Teresa De Jesus
dc.contributor.authorHernández Pérez, Sara
dc.contributor.authorMattila, Pieta K.
dc.contributor.authorYuseff Sepúlveda, María Isabel
dc.date.accessioned2023-11-10T16:17:27Z
dc.date.available2023-11-10T16:17:27Z
dc.date.issued2023
dc.description.abstractThe capacity of B cells to extract immobilized antigens through the formation of an immune synapse can be tuned by the physical characteristics of the surface where antigens are encountered. However, the underlying mechanisms that couple mechanosensing by B cells to antigen extraction and processing remain poorly understood.We show that B cells activated by antigens associated with stiffer substrates exhibit enhanced spreading responses and higher tubulin acetylation at the center of the immune synapse, where less motile lysosomes preferentially localize. This process is coupled to the translocation of the microtubule acetylase, ATAT1 to the cytoplasm of B cells, which occurs as a mechano-response during BCR stimulation. Accordingly, B cells silenced for ATAT1 are unable to stabilize lysosomes at the synaptic interface and display a lower capacity to extract and present immobilized antigens to T cells.Overall, these findings highlight how BCR-dependent mechano-responses trigger microtubule network modifications to precisely orchestrate lysosome positioning to promote antigen extraction and presentation in B cells
dc.fechaingreso.objetodigital2023-11-10
dc.fuente.origenORCID
dc.identifier.doi10.1101/2023.08.28.555091
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1101/2023.08.28.555091
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75279
dc.information.autorucFacultad de Ciencias Biológicas; Del Valle Batalla, Felipe Andrés; 0000-0001-6113-9549; 220963
dc.information.autorucFacultad de Ciencias Biológicas; Riobo Cavada, Isidora Teresa De Jesus; S/I; 1066783
dc.information.autorucFacultad de Ciencias Biológicas; Yuseff Sepúlveda, María Isabel; 0000-0001-8172-3785; 229802
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final30
dc.pagina.inicio1
dc.revistabioRxiv
dc.rightsacceso abierto
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleSpatiotemporal control of microtubule acetylation by mechanical cues regulates lysosome dynamics at the immune synapse of B cells to promote antigen presentation
dc.typepreprint
sipa.codpersvinculados220963
sipa.codpersvinculados1066783
sipa.codpersvinculados229802
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