Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

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dc.contributor.authorVeloso Gimenez, Valentina
dc.contributor.authorEscamilla, Rosalba
dc.contributor.authorNecuñir, David
dc.contributor.authorCorrales Orovio, Rocio
dc.contributor.authorRiveros, Sergio
dc.contributor.authorMarino, Carlo
dc.contributor.authorEhrenfeld, Carolina
dc.contributor.authorGuzman, Christian Dani
dc.contributor.authorBoric, Mauricio P.
dc.contributor.authorRebolledo, Rolando
dc.contributor.authorEgaña, José Tomas
dc.date.accessioned2024-01-10T13:44:53Z
dc.date.available2024-01-10T13:44:53Z
dc.date.issued2021
dc.description.abstractOxygen is the key molecule for aerobic metabolism, but no animal cells can produce it, creating an extreme dependency on external supply. In contrast, microalgae are photosynthetic microorganisms, therefore, they are able to produce oxygen as plant cells do. As hypoxia is one of the main issues in organ transplantation, especially during preservation, the main goal of this work was to develop the first generation of perfusable photosynthetic solutions, exploring its feasibility for ex vivo organ preservation. Here, the microalgae Chlamydomonas reinhardtii was incorporated in a standard preservation solution, and key aspects such as alterations in cell size, oxygen production and survival were studied. Osmolarity and rheological features of the photosynthetic solution were comparable to human blood. In terms of functionality, the photosynthetic solution proved to be not harmful and to provide sufficient oxygen to support the metabolic requirement of zebrafish larvae and rat kidney slices. Thereafter, isolated porcine kidneys were perfused, and microalgae reached all renal vasculature, without inducing damage. After perfusion and flushing, no signs of tissue damage were detected, and recovered microalgae survived the process. Altogether, this work proposes the use of photosynthetic microorganisms as vascular oxygen factories to generate and deliver oxygen in isolated organs, representing a novel and promising strategy for organ preservation.
dc.description.funderCORFO grant
dc.description.funderNational Agency for Research and Development (ANID) PhD Scholarship Program
dc.description.funderANID PAI program
dc.description.funderFONDECYT grant
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3389/fbioe.2021.796157
dc.identifier.issn2296-4185
dc.identifier.pubmedidMEDLINE:34976984
dc.identifier.urihttps://doi.org/10.3389/fbioe.2021.796157
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78950
dc.identifier.wosidWOS:000738722700001
dc.information.autorucEscuela de ingeniería ; Veloso Gimenez, Valentina ; S/I ; 1049917
dc.information.autorucFacultad de ciencias biológicas ; Escamilla, Rosalba ; S/I ; 250321
dc.information.autorucEscuela de medicina ; Riveros, Sergio ; S/I ; 178359
dc.information.autorucEscuela de medicina ; Marino, Carlo ; S/I ; 199753
dc.information.autorucFacultad de ciencias biológicas ; Ehrenfeld, Carolina ; S/I ; 250108
dc.information.autorucFacultad de ciencias biológicas ; Boric, Mauricio P. ; S/I ; 56355
dc.information.autorucInstituto de ingeniería biológica y médica ; Rebolledo, Rolando ; 0000-0003-3501-4042 ; 127520
dc.information.autorucInstituto de ingeniería biológica y médica ; Egaña, José Tomas ; S/I ; 1018715
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherFrontiers Media SA
dc.revistaFrontiers in Bioengineering and Biotechnology
dc.rightsacceso abierto
dc.subjectOrgan preservation
dc.subjectOrgan perfusion
dc.subjectIschemia
dc.subjectHypoxia
dc.subjectPhotosynthetic microorganisms
dc.subjectChlamydomonas reinhardtii
dc.subjectPhotosynthesis
dc.subjectMachine perfusion
dc.subjectBiomaterials
dc.subjectZebrafish
dc.subjectTherapy
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDevelopment of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation
dc.typeartículo
dc.volumen9
sipa.codpersvinculados1049917
sipa.codpersvinculados250321
sipa.codpersvinculados178359
sipa.codpersvinculados199753
sipa.codpersvinculados250108
sipa.codpersvinculados56355
sipa.codpersvinculados127520
sipa.codpersvinculados1018715
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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