Reprimo as a Potential Biomarker for Early Detection in Gastric Cancer

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dc.contributor.authorBernal, Carolina
dc.contributor.authorAguayo, Francisco
dc.contributor.authorVillarroel, Cynthia
dc.contributor.authorVargas, Macarena
dc.contributor.authorDiaz, Ignacio
dc.contributor.authorOssandon, Francisco J.
dc.contributor.authorSantibanez, Eudocia
dc.contributor.authorPalma, Mariana
dc.contributor.authorAravena, Edmundo
dc.contributor.authorBarrientos, Carlos
dc.contributor.authorCorvalan, Alejandro H.
dc.date.accessioned2024-01-10T14:21:33Z
dc.date.available2024-01-10T14:21:33Z
dc.date.issued2008
dc.description.abstractPurpose: Gastric cancer is a curable disease if diagnosed at early stage. However, most cases are diagnosed at advanced stage because of the lack of screening programs. Therefore, the identification of plasma biomarkers for early detection is necessary.
dc.description.abstractExperimental Design: To search for these biomarkers, we evaluated the DNA methylation patterns of 24 genes by Methylation-specific PCR in primary tissues from 32 retrospectively collected gastric cancer cases (testing group). Correlation between methylation and gene expression was evaluated in the MKN-45 cell line after treatment with 5-aza-2'-deoxycytidine. The most frequently hypermethylated genes were next evaluated in primary tissues and plasma samples from 43 prospectively collected gastric cancer cases as well as plasma samples from 31 asymptomatic age- and gender-matched controls (validation group).
dc.description.abstractResults: In the testing group, 11 genes were hypermethylated in at least 50% of cases (APC, SHP1, E-cadherin, ER, Reprimo, SEMA3B, 3OST2, p14, p15, DAPK, and p16). Eight genes (BRCA1, p73, RAR beta, hMLH1, RIZI, RUNX3, MGMT, and TIMP3) were statistically associated with a particular variant of gastric cancer, the signet-ring cell type (P = 0.03). Seven genes (APC, SHP1, E-cadherin, ER, Reprimo, SEMA3B, and 3OST2) were next evaluated in the validation group. We confirm the high frequency of methylation in primary tumors for all seven genes. However, only APC and Reprimo were frequently methylated in pair plasma samples. In asymptomatic controls, only Reprimo was infrequently methylated in comparison with plasma from gastric cancer cases (P < 0.001).
dc.description.abstractConclusion: Our results identified specific methylation profile associated to signet-ring cell-type histology and aberrant hypermethylation of Reprimo as a potential biomarker for early detection of gastric cancer.
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1158/1078-0432.CCR-07-4522
dc.identifier.eissn1557-3265
dc.identifier.issn1078-0432
dc.identifier.pubmedidMEDLINE:18829507
dc.identifier.urihttps://doi.org/10.1158/1078-0432.CCR-07-4522
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79707
dc.identifier.wosidWOS:000260142500041
dc.information.autorucMedicina;Aguayo, F.;S/I;1005475
dc.information.autorucCiencias Biológicas;Bernal, C.;S/I;1003963
dc.information.autorucMedicina;Corvalán, AH.;S/I;63885
dc.information.autorucCiencias Biológicas;Diaz, I.;S/I;142355
dc.information.autorucMedicina;Santibanez, E.;S/I;1004649
dc.information.autorucCiencias Biológicas;Vargas, M.;S/I;169816
dc.information.autorucCiencias Biológicas;Villarroel, C.;S/I;132633
dc.issue.numero19
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final6269
dc.pagina.inicio6264
dc.publisherAMER ASSOC CANCER RESEARCH
dc.revistaCLINICAL CANCER RESEARCH
dc.rightsacceso restringido
dc.subjectCPG ISLAND METHYLATION
dc.subjectTUMOR-RELATED GENES
dc.subjectSIGNET-RING CELL
dc.subjectPROMOTER HYPERMETHYLATION
dc.subjectDNA METHYLATION
dc.subjectMICROSATELLITE INSTABILITY
dc.subjectGALLBLADDER CARCINOMA
dc.subjectABERRANT METHYLATION
dc.subjectHUMAN MALIGNANCIES
dc.subjectSUPPRESSOR GENES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleReprimo as a Potential Biomarker for Early Detection in Gastric Cancer
dc.typeartículo
dc.volumen14
sipa.codpersvinculados1005475
sipa.codpersvinculados1003963
sipa.codpersvinculados63885
sipa.codpersvinculados142355
sipa.codpersvinculados1004649
sipa.codpersvinculados169816
sipa.codpersvinculados132633
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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